在肝脏来源的蛋白聚糖存在的情况下形成的成年大鼠肝细胞多细胞球体持续产生高白蛋白。
Continued high albumin production by multicellular spheroids of adult rat hepatocytes formed in the presence of liver-derived proteoglycans.
作者信息
Koide N, Shinji T, Tanabe T, Asano K, Kawaguchi M, Sakaguchi K, Koide Y, Mori M, Tsuji T
机构信息
First Department of Internal Medicine, Okayama University Medical School, Shikata-cho, Japan.
出版信息
Biochem Biophys Res Commun. 1989 May 30;161(1):385-91. doi: 10.1016/0006-291x(89)91609-4.
Adult rat hepatocytes formed floating multicellular spheroids, when they were cultured with proteoglycan fraction isolated from rat liver reticulin fibers. Cells in the spheroid showed only low growth activity. Albumin production by the spheroids increased up to 1.5 micrograms/micrograms DNA/day (180 micrograms/mg Protein/day) during the first 6 days and remained constant thereafter. In contrast, the albumin production by the monolayer markedly decreased after 4 days. The spheroid culture appears to be more suitable than the monolayer in studying differentiated functions of adult hepatocytes.
成年大鼠肝细胞在与从大鼠肝脏网状纤维中分离出的蛋白聚糖组分一起培养时，会形成漂浮的多细胞球体。球体中的细胞仅表现出较低的生长活性。在最初的6天里，球体产生白蛋白的量增加到1.5微克/微克DNA/天（180微克/毫克蛋白质/天），此后保持恒定。相比之下，单层培养的肝细胞在4天后白蛋白产量显著下降。在研究成年肝细胞的分化功能方面，球体培养似乎比单层培养更合适。
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