共固定化细菌生物发光酶的光纤传感器。

Fibre-optic sensor with co-immobilised bacterial bioluminescence enzymes.

作者信息

Gautier S M, Blum L J, Coulet P R

机构信息

Laboratoire de Génie Enzymatique--Atelier de Biotechnologie, CNRS--Université Claude Bernard Lyon, Villeurbanne, France.

出版信息

Biosensors. 1989;4(3):181-94. doi: 10.1016/0265-928x(89)80019-7.

DOI:10.1016/0265-928x(89)80019-7

PMID:2730686

Abstract

A fibre-optic bioluminescent sensor for the microdetermination of NADH is described. Measurements can be performed in the linear range 1 x 10(-9) M-3 x 10(-6) M with a detection limit of 3 x 10(-10) M using the bacterial luciferase and NAD(P)H:FMN oxidoreductase co-immobilised on a preactivated polyamide membrane. The relative standard deviation was 4.8-5.5% at 4 x 10(-8) M NADH and the steady-state response time was 2 min. When stored at -20 degrees C with 20% glycerol, the activity of the bioactive membranes was higher than that measured prior to freezing and then remained stable for more than four months.

摘要

描述了一种用于微量测定NADH的光纤生物发光传感器。使用共固定在预活化聚酰胺膜上的细菌荧光素酶和NAD(P)H:FMN氧化还原酶，可在1×10(-9)M - 3×10(-6)M的线性范围内进行测量，检测限为3×10(-10)M。在4×10(-8)M NADH时，相对标准偏差为4.8 - 5.5%，稳态响应时间为2分钟。当在-20℃下用20%甘油储存时，生物活性膜的活性高于冷冻前测量的活性，并且在四个多月内保持稳定。