Rangel L F, Rocha S, Casal G, Castro R, Severino R, Azevedo C, Cavaleiro F, Santos M J
Laboratory of Animal Pathology, Department of Biology, Faculty of Sciences, University of Porto, Porto, Portugal.
Interdisciplinary Centre of Marine and Environmental Research (CIIMAR/CIMAR), University of Porto, Porto, Portugal.
J Fish Dis. 2017 Feb;40(2):243-262. doi: 10.1111/jfd.12508. Epub 2016 Jun 16.
Ortholinea labracis n. sp. is described and its life cycle is inferred from a Southern Portuguese fish farm, with basis on microscopic and molecular procedures. This myxosporean parasite infects the urinary bladder of the European seabass Dicentrarchus labrax and the intestinal epithelium of a marine oligochaete of the genus Tectidrilus. Myxospores subspherical in valvular view and ellipsoidal in sutural view measuring 7.6 ± 0.3 (6.8-8.7) μm in length, 7.2 ± 0.2 (6.7-7.7) μm in width and 6.5 ± 0.4 (5.8-7.7) μm in thickness. Two polar capsules, 3.0 ± 0.2 (2.6-3.4) μm long and 2.4 ± 0.1 (2.0-2.9) μm wide, located at the same level, but with divergent orientation and opening to opposite sides of the suture line. Sequencing of the SSU rRNA gene revealed a similarity of 100% between the analysed myxospores and triactinomyxon actinospores. The phylogenetic setting of O. labracis n. sp. shows subgrouping in correlation with tissue tropism, but identifies this parasite as another exception to the main division of Myxosporea into the main freshwater and marine lineages.
描述了新种Ortholinea labracis，并基于显微镜和分子程序，从葡萄牙南部的一个养鱼场推断出其生命周期。这种粘孢子虫寄生虫感染欧洲海鲈（Dicentrarchus labrax）的膀胱和Tectidrilus属海洋寡毛纲动物的肠上皮。粘孢子虫在瓣面观为近球形，在缝线观为椭圆形，长7.6±0.3（6.8 - 8.7）μm，宽7.2±0.2（6.7 - 7.7）μm，厚6.5±0.4（5.8 - 7.7）μm。两个极囊，长3.0±0.2（2.6 - 3.4）μm，宽2.4±0.1（2.0 - 2.9）μm，位于同一水平，但方向不同，向缝线的相反两侧开口。SSU rRNA基因测序显示，分析的粘孢子虫与三放射孢子虫的放射孢子之间的相似度为100%。Ortholinea labracis新种的系统发育定位显示，与组织嗜性相关的亚群划分，但将这种寄生虫确定为粘孢子虫主要分为淡水和海洋主要谱系的另一个例外。
