Suhaimi Nadhirah Syafiqah, Sellyei Boglárka, Udvari Zsolt, Székely Csaba, Cech Gábor
HUN-REN Veterinary Medical Research Institute, Budapest, Hungary.
Doctoral School of Animal Biotechnology and Animal Science, Hungarian University of Agriculture and Life Sciences, Gödöllő, Hungary.
Int J Parasitol Parasites Wildl. 2024 Sep 18;25:100994. doi: 10.1016/j.ijppaw.2024.100994. eCollection 2024 Dec.
Six distinct actinospore types were identified in the intestinal epithelium of oligochaetes collected from the Szigetbecse and Makád fish farms of Ráckeve Danube Arm Fishing Association, in Hungary. Four new types: triactinomyxon type, raabeia type, aurantiactinomyxon type 1, and aurantiactinomyxon type 2, were described morphologically and molecularly from three invertebrate host species: , and . The 18S ribosomal DNA (rDNA) analysis revealed that these new types of actinospores did not match any myxospore sequences available in GenBank. Phylogenetic analysis placed the triactinomyxon type within the clade that parasitizes cyprinid fish, while the raabeia type and aurantiactinomyxon type 2 were both placed within the clade associated with Perciformes fish. Aurantiactinomyxon type 1 was clustered in a clade containing gill-infecting spp. from Esociformes fish. However, no myxospores have been found yet to link to the newly sequenced actinospores reported in this survey. This study also reports the first occurrence of two oligochaetes species, and in Hungary, specifically in fish farms of Ráckeve Danube Arm Fishing Association. Moreover, this is the first report on the involvement of in the life cycle of myxozoans.
在从匈牙利拉克韦多瑙河支流捕鱼协会的希盖特贝切和马卡德养鱼场采集的寡毛纲动物的肠上皮中，鉴定出了六种不同类型的放射孢子。从三种无脊椎动物宿主物种中，对四种新类型：三放射孢子虫型、拉贝亚型、橙黄放射孢子虫1型和橙黄放射孢子虫2型进行了形态学和分子学描述。18S核糖体DNA（rDNA）分析表明，这些新型放射孢子与GenBank中现有的任何粘孢子序列均不匹配。系统发育分析表明，三放射孢子虫型位于寄生于鲤科鱼类的进化枝内，而拉贝亚型和橙黄放射孢子虫2型均位于与鲈形目鱼类相关的进化枝内。橙黄放射孢子虫1型聚集在一个包含来自狗鱼目鱼类的鳃感染粘体虫属物种的进化枝中。然而，尚未发现与本次调查中报道的新测序放射孢子相关的粘孢子。本研究还报道了两种寡毛纲动物物种在匈牙利首次出现，特别是在拉克韦多瑙河支流捕鱼协会的养鱼场中。此外，这是关于 参与粘孢子虫生命周期的首次报道。
