Department of Obstetrics, Guangzhou Women and Children's Medical Center, Guangzhou medical University, Guangzhou, China.
Department of Physiology, Guangzhou medical University, Guangzhou, China.
Hypertens Res. 2016 Oct;39(10):701-708. doi: 10.1038/hr.2016.53. Epub 2016 Jun 16.
Preeclampsia (PE), one of the most common disorders of pregnancy, is characterized by hypertension and albuminuria. In severe cases, PE results in eclampsia-like seizures. Studies have suggested that severe PE is related to an exaggerated systemic inflammatory response, which may increase sensitivity to seizures. In the current study, we investigated whether the seizure activity of neurons was enhanced under excessive systemic inflammation. We also sought to determine whether MgSO could reduce the effects of systemic inflammation on seizure activity after electrical stimulation in a lipopolysaccharide (LPS)-induced model of PE. In addition to pregnancy outcomes, we analyzed biochemical parameters to ascertain whether our PE model was successful. Enzyme-linked immunosorbent assay analysis revealed that the levels of inflammatory cytokines (tumor necrosis factor (TNF)-α and interleukin (IL)-1β) were significantly higher in the LPS-treated rats than in the untreated rats. After electrical stimulation, behavioral assessments showed that the LPS-treated rats that were not treated with MgSO had the shortest latency period to develop a seizure and the longest seizure duration. The electroencephalographic (EEG) recordings in the hippocampus demonstrated that this group also had the highest EEG amplitude. MgSO treatment significantly decreased both TNF-α and IL-1β concentrations, increased the latency to develop a seizure, decreased the seizure duration and shortened the EEG amplitude. These results suggest that neuronal seizure activity and systemic inflammation are increased in severe PE. In addition, MgSO treatment reduced systemic inflammation and seizure severity. We conclude that excessive systemic inflammation in PE promotes eclampsia seizures, which can be attenuated by MgSO treatment.
子痫前期（PE）是最常见的妊娠并发症之一，其特征是高血压和蛋白尿。在严重的情况下，PE 会导致类似子痫的抽搐。研究表明，严重的 PE 与全身性炎症反应过度有关，这可能会增加对抽搐的敏感性。在本研究中，我们研究了在过度全身性炎症下神经元的抽搐活动是否增强。我们还试图确定硫酸镁是否可以减轻 LPS 诱导的 PE 模型中电刺激后全身性炎症对抽搐活动的影响。除了妊娠结局，我们还分析了生化参数以确定我们的 PE 模型是否成功。酶联免疫吸附试验分析显示，LPS 处理的大鼠的炎症细胞因子（肿瘤坏死因子（TNF）-α和白细胞介素（IL）-1β）水平明显高于未处理的大鼠。电刺激后，行为评估显示，未用硫酸镁治疗的 LPS 处理大鼠出现抽搐的潜伏期最短，抽搐持续时间最长。海马的脑电图（EEG）记录显示，该组的 EEG 幅度也最高。硫酸镁治疗显著降低了 TNF-α和 IL-1β的浓度，增加了发生抽搐的潜伏期，降低了抽搐持续时间，并缩短了 EEG 幅度。这些结果表明，严重的 PE 中神经元抽搐活动和全身性炎症增加。此外，硫酸镁治疗减轻了全身性炎症和抽搐的严重程度。我们得出结论，PE 中的过度全身性炎症促进子痫发作，硫酸镁治疗可减轻这种发作。
