用蜱唾液腺提取物对牛进行免疫接种。

Immunization of Cattle with Tick Salivary Gland Extracts.

作者信息

Nikpay Ali, Nabian Sedigheh

机构信息

Department of Pathobiology, Amol University of Special Modern Technologies, Amol, Iran.

Department of Parasitology, Faculty of Veterinary Medicine, Tehran, Iran.

出版信息

J Arthropod Borne Dis. 2016 Jan 6;10(3):281-90. eCollection 2016 Sep.

PMID:27308287

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4906735/

Abstract

BACKGROUND

Rhipicephalus (Boophilus) annulatus tick is one of the most important ectoparasite of cattle. Recently, several laboratories in the world have been concentrated on immunizing cattle against tick using various types of tissue extracts of ticks. The aim of this study was to evaluate the effect of immunization of cattle with tick salivary gland extract on biological parameters of ticks and humoral immune responses of cattle.

METHODS

Fourteen more dominant protein bands identified as immunogenic by Western-blot analysis were eluted from polyacrylamide gel. Test and control groups were injected three times with eluted proteins and sterile PBS (pH= 7.2) respectively with equivalent amount of adjuvant. After four weeks a tick challenge was performed. Finally, biological parameters of collected engorged female ticks were recorded and humoral immune responses to immunization measured by ELISA.

RESULTS

The results indicated immunization of cattle resulted in reduction in mean tick counts, attachment, engorgement weights, feeding index, egg mass weight, hatchability and fertility index (respectively 63.1%, 62.6%, 30.2%, 36.4%, 40%, 78.7% and 13.3%) and increased duration of feeding, preoviposition and incubation period of eggs (respectively 8.6%, 45 and 31.34%). All changes were statistically significant (P< 0.05). Results showed an increase in antibody production of test group from the first week after immunization. The antibody level was boosted following tick infestation.

CONCLUSION

This investigation indicates that immunization of cattle with these antigens could induce a protective immune response against Rh. (B.) annulatus tick that would be expected to provide a safe non-chemical means of tick control.

摘要

背景

微小牛蜱是牛最重要的体表寄生虫之一。最近，世界上几个实验室一直致力于使用蜱的各种组织提取物对牛进行抗蜱免疫。本研究的目的是评估用蜱唾液腺提取物免疫牛对蜱的生物学参数和牛的体液免疫反应的影响。

方法

通过蛋白质印迹分析鉴定为具有免疫原性的14条更多主要蛋白带从聚丙烯酰胺凝胶中洗脱。试验组和对照组分别用洗脱的蛋白质和无菌磷酸盐缓冲液（pH = 7.2）注射三次，每次注射等量的佐剂。四周后进行蜱叮咬攻击。最后，记录收集的饱血雌蜱的生物学参数，并通过酶联免疫吸附测定法测量对免疫的体液免疫反应。

结果

结果表明，牛的免疫导致平均蜱数、附着率、饱血重量、摄食指数、卵块重量、孵化率和繁殖指数降低（分别为63.1%、62.6%、30.2%、36.4%、40%、78.7%和13.3%），并延长了摄食持续时间、产卵前期和卵的孵化期（分别为8.6%、45和31.34%）。所有变化均具有统计学意义（P<0.05）。结果显示，试验组在免疫后的第一周抗体产生增加。蜱侵袭后抗体水平升高。