Sereflican Murat, Yurttas Veysel, Ozyalvacli Gulzade, Terzi Elcin Hakan, Turkoglu Sule Aydin, Yildiz Serpil, Ilgaz Yasin, Seyhan Sinan, Oral Mesut, Dagli Muharrem
Department of Otolaryngology, Abant Izzet Baysal University, Faculty of Medicine, Bolu, Turkey.
Department of Otolaryngology, Abant Izzet Baysal University, Faculty of Medicine, Bolu, Turkey.
Am J Otolaryngol. 2016 Sep-Oct;37(5):407-15. doi: 10.1016/j.amjoto.2016.05.006. Epub 2016 May 24.
We aimed to determine the effects of methylprednisolone and thymoquinone on nerve healing in a traumatic facial nerve paralysis animal model.
Twenty-four rabbits were randomly divided into 4 groups: group I: control group received no medication and no trauma; group II: sham group received no medication after facial nerve trauma group III: 5mg/kg/day thymoquinone administered; group IV: 1mg/kg/day methylprednisolone administered. An initial electrophysiological assessment was performed in all the animals. The buccal branch of the facial nerve was then clipped to form a traumatic facial paralysis model. The drugs were administered for two weeks once a day. At the end of the second month, the electrophysiological assessments were performed and the distal part of the traumatic facial nerve were dissected and examined under light microscopy.
Best nerve regeneration was observed in the control and the thymoquinone groups, respectively, whereas the weakest regeneration was determined in the sham group. Thymoquinone and methylprednisolone significantly increased nerve recovery, as measured by histopathological scores and electrophysiological assessment. In the thymoquinone group, due to postoperative amplitude, axon diameter and thickness of myelin sheath values were significantly further increased nerve regeneration compared to that of the methylprednisolone group and these values were close to those of the values of the control group.
Thymoquinone was slightly better than methylprednisolone for functional nerve recovery. The neuroprotective effect of thymoquinone was attributed to its antioxidant and anti-inflammatory effects. Thymoquinone can have a new treatment option to ameliorate the nerve injury.
我们旨在确定甲基强的松龙和百里醌对创伤性面神经麻痹动物模型中神经愈合的影响。
24只兔子随机分为4组：第一组：对照组，未接受药物治疗且未遭受创伤；第二组：假手术组，面神经创伤后未接受药物治疗；第三组：给予5mg/kg/天的百里醌；第四组：给予1mg/kg/天的甲基强的松龙。对所有动物进行了初始电生理评估。然后夹闭面神经颊支以形成创伤性面神经麻痹模型。每天给药一次，持续两周。在第二个月末，进行电生理评估，并解剖创伤性面神经的远端部分，在光学显微镜下检查。
分别在对照组和百里醌组观察到最佳的神经再生，而在假手术组中确定再生最弱。通过组织病理学评分和电生理评估测量，百里醌和甲基强的松龙显著提高了神经恢复。在百里醌组中，由于术后振幅、轴突直径和髓鞘厚度值，与甲基强的松龙组相比，神经再生显著进一步增加，并且这些值接近对照组的值。
在功能性神经恢复方面，百里醌略优于甲基强的松龙。百里醌的神经保护作用归因于其抗氧化和抗炎作用。百里醌可为改善神经损伤提供一种新的治疗选择。
