Niu Ying, Qureshi Jawwad A, Ni Xinzhi, Head Graham P, Price Paula A, Meagher Robert L, Kerns David, Levy Ronnie, Yang Xiangbing, Huang Fangneng
Department of Entomology, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA.
Department of Entomology and Nematology, University of Florida, IFAS SWFREC, Immokalee, FL 34142, USA.
J Invertebr Pathol. 2016 Jul;138:66-72. doi: 10.1016/j.jip.2016.06.005. Epub 2016 Jun 14.
The fall armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae), is a target pest of transgenic maize and cotton expressing Bacillus thuringiensis (Bt) proteins in both North and South America. In 2013 and 2014, a total of 215 F2 two-parent families of S. frugiperda were established using single-pair mating of field individuals collected from seven locations in four states of the southern U.S.: Texas, Louisiana, Georgia, and Florida. The objective of the investigation was to detect resistance alleles in field populations to Cry2Ab2, a common Bt protein produced in transgenic maize and cotton. For each F2 family, 128 F2 neonates were screened on leaf tissue of Cry2Ab2 maize plants in the laboratory. A conservative estimate of the frequency of major Cry2Ab2 resistance alleles in S. frugiperda from the four states was 0.0023 with a 95% credibility interval of 0.0003-0.0064. In addition, six families were considered to likely possess minor resistance alleles at a frequency of 0.0082 with a 95% credibility interval of 0.0033-0.0152. One F2 family from Georgia (GA-15) was confirmed to possess a major resistance allele to the Cry2Ab2 protein. Larvae from this family survived well on whole maize plants expressing Cry2Ab2 protein and demonstrated a significant level (>15-fold) of resistance when fed with the same protein incorporated in a meridic diet. The detection of the major resistance allele along with the relatively abundant minor resistance alleles revealed in this study may have important implications for resistance management.
草地贪夜蛾，即草地贪夜蛾（J. E. 史密斯）（鳞翅目：夜蛾科），是在北美洲和南美洲对表达苏云金芽孢杆菌（Bt）蛋白的转基因玉米和棉花构成危害的一种害虫。2013年和2014年，利用从美国南部四个州（得克萨斯州、路易斯安那州、佐治亚州和佛罗里达州）七个地点采集的田间个体进行单对交配，共建立了215个草地贪夜蛾F2双亲家庭。该调查的目的是检测田间种群中对Cry2Ab2（转基因玉米和棉花中产生的一种常见Bt蛋白）的抗性等位基因。对于每个F2家庭，在实验室中对128只F2初孵幼虫在Cry2Ab2玉米植株的叶片组织上进行筛选。来自这四个州的草地贪夜蛾中主要Cry2Ab2抗性等位基因频率的保守估计为0.0023，95%可信区间为0.0003 - 0.0064。此外，有六个家庭被认为可能携带频率为0.0082的次要抗性等位基因，95%可信区间为0.0033 - 0.0152。来自佐治亚州的一个F2家庭（GA - 15）被证实携带对Cry2Ab2蛋白的主要抗性等位基因。该家庭的幼虫在表达Cry2Ab2蛋白的整株玉米上存活良好，并且在以添加相同蛋白的人工饲料喂养时表现出显著水平（>15倍）的抗性。本研究中检测到的主要抗性等位基因以及相对丰富的次要抗性等位基因可能对抗性管理具有重要意义。
