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智能手机代谢组学平台在五味子鉴定中的应用。

Application of a Smartphone Metabolomics Platform to the Authentication of Schisandra sinensis.

作者信息

Kwon Hyuk Nam, Phan Hong-Duc, Xu Wen Jun, Ko Yoon-Joo, Park Sunghyouk

机构信息

College of Pharmacy, Natural Product Research Institute, Seoul National University, Sillim-dong, Gwanak-gu, Seoul, Korea.

National Centre for Inter-University Research Facilities (NCIRF), Seoul National University, Sillim-dong, Gwanak-gu, Seoul, Korea.

出版信息

Phytochem Anal. 2016 May;27(3-4):199-205. doi: 10.1002/pca.2617.

Abstract

INTRODUCTION

Herbal medicines have been used for a long time all around the world. Since the quality of herbal preparations depends on the source of herbal materials, there has been a strong need to develop methods to correctly identify the origin of materials.

OBJECTIVE

To develop a smartphone metabolomics platform as a simpler and low-cost alternative for the identification of herbal material source.

METHODOLOGY

Schisandra sinensis extracts from Korea and China were prepared. The visible spectra of all samples were measured by a smartphone spectrometer platform. This platform included all the necessary measures built-in for the metabolomics research: data acquisition, processing, chemometric analysis and visualisation of the results. The result of the smartphone metabolomics platform was compared to that of NMR-based metabolomics, suggesting the feasibility of smartphone platform in metabolomics research.

RESULTS

The smartphone metabolomics platform gave similar results to the NMR method, showing good separation between Korean and Chinese materials and correct predictability for all test samples.

CONCLUSION

With its accuracy and advantages of affordability, user-friendliness, and portability, the smartphone metabolomics platform could be applied to the authentication of other medicinal plants. Copyright © 2016 John Wiley & Sons, Ltd.

摘要

引言

草药在世界各地已被使用很长时间。由于草药制剂的质量取决于草药原料的来源,因此迫切需要开发能够正确识别原料来源的方法。

目的

开发一种智能手机代谢组学平台,作为一种更简单且低成本的识别草药原料来源的替代方法。

方法

制备了来自韩国和中国的五味子提取物。所有样品的可见光谱通过智能手机光谱仪平台进行测量。该平台包含代谢组学研究所需的所有内置措施:数据采集、处理、化学计量分析以及结果可视化。将智能手机代谢组学平台的结果与基于核磁共振(NMR)的代谢组学结果进行比较,表明智能手机平台在代谢组学研究中的可行性。

结果

智能手机代谢组学平台给出了与核磁共振方法相似的结果,显示出韩国和中国原料之间的良好分离以及对所有测试样品的正确预测能力。

结论

智能手机代谢组学平台具有准确性以及价格实惠、用户友好和便携的优点,可应用于其他药用植物的鉴定。版权所有© 2016约翰威立父子有限公司。

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