Luo Liyun, Chen Bairong, Li Songbiao, Wei Xiaoliang, Liu Tianmin, Huang Yin, Lin Xiufang
Department of Cardiology, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, Guangdong 519000, China.
Dis Markers. 2016;2016:3841927. doi: 10.1155/2016/3841927. Epub 2016 May 22.
Aims. MicroRNAs (miRNAs) are involved in the pathogenesis of coronary artery disease (CAD). The objective of this study is to determine plasma levels of miR-10a in CAD and analyze its association with the severity of CAD. Materials and Methods. Plasma miR-10a levels in 60 CAD patients including stable angina pectoris (SAP) (n = 29), unstable angina pectoris (UAP) or non-ST elevation myocardial infarction (MI) (NSTEMI) (n = 17), or ST elevation MI (STEMI) (n = 14) and 20 non-CAD subjects were assessed by real-time polymerase chain reaction (qRT-PCR), and associations of miR-10a levels with risk factors of CAD and its severity were analyzed. Results. The qRT-PCR results showed that plasma miR-10a levels were decreased in CAD patients, and CAD with high SYNTAX scores or STEMI was significantly associated with lower miR-10a levels. Conclusions. Lower plasma miR-10a levels were negatively associated with the presence as well as severity of CAD, and plasma miR-10a can act as a potential biomarker for estimating the presence and severity of CAD.
目的。微小RNA（miRNA）参与冠状动脉疾病（CAD）的发病机制。本研究的目的是测定CAD患者血浆中miR-10a的水平，并分析其与CAD严重程度的关联。材料与方法。通过实时聚合酶链反应（qRT-PCR）评估60例CAD患者（包括稳定型心绞痛（SAP）患者29例、不稳定型心绞痛（UAP）或非ST段抬高型心肌梗死（MI）（NSTEMI）患者17例、ST段抬高型心肌梗死（STEMI）患者14例）及20例非CAD受试者血浆中miR-10a的水平，并分析miR-10a水平与CAD危险因素及其严重程度的关联。结果。qRT-PCR结果显示，CAD患者血浆miR-10a水平降低，且SYNTAX评分高的CAD或STEMI与较低的miR-10a水平显著相关。结论。血浆miR-10a水平降低与CAD的存在及其严重程度呈负相关，血浆miR-10a可作为评估CAD存在及严重程度的潜在生物标志物。
