Kim Tae-Ho, Park Eui Kyun, Huh Man-Il, Kim Hong Kyun, Kim Shin-Yoon, Lee Sang-Han
Biomedical Research Institute, Kyungpook National University Hospital, Daegu 41940, Republic of Korea; Skeletal Diseases Genome Research Center, Kyungpook National University Hospital, Daegu 41940, Republic of Korea.
Department of Oral Pathology and Regenerative Medicine, School of Dentistry, IHBR, Kyungpook National University, Daegu 41940, Republic of Korea.
Evid Based Complement Alternat Med. 2016;2016:3284704. doi: 10.1155/2016/3284704. Epub 2016 May 30.
Inhibition of osteoclast differentiation and bone resorption is a therapeutic strategy for the management of postmenopausal bone loss. This study investigated the effects of Rhus javanica (R. javanica) extracts on bone marrow cultures to develop agents from natural sources that may prevent osteoclastogenesis. Extracts of R. javanica (eGr) cocoons spun by Rhus javanica (Bell.) Baker inhibited the osteoclast differentiation and bone resorption. The effects of aqueous extract (aeGr) or 100% ethanolic extract (eeGr) on ovariectomy- (OVX-) induced bone loss were investigated by various biochemical assays. Furthermore, microcomputed tomography (µCT) was performed to study bone remodeling. Oral administration of eGr (30 mg or 100 mg/kg/day for 6 weeks) augmented the inhibition of femoral bone mineral density (BMD), bone mineral content (BMC), and other factors involved in bone remodeling when compared to OVX controls. Additionally, eGr slightly decreased bone turnover markers that were increased by OVX. Therefore, it may be suggested that the protective effects of eGr could have originated from the suppression of OVX-induced increase in bone turnover. Collectively, the findings of this study indicate that eGr has potential to activate bone remodeling by inhibiting osteoclast differentiation and bone loss.
抑制破骨细胞分化和骨吸收是治疗绝经后骨质流失的一种策略。本研究调查了盐肤木提取物对骨髓培养物的影响，以开发可能预防破骨细胞生成的天然来源药物。盐肤木（Rhus javanica (Bell.) Baker）所结的盐肤木提取物（eGr）抑制破骨细胞分化和骨吸收。通过各种生化测定法研究了水提取物（aeGr）或100%乙醇提取物（eeGr）对去卵巢（OVX）诱导的骨质流失的影响。此外，进行了微计算机断层扫描（µCT）以研究骨重塑。与OVX对照组相比，口服eGr（30毫克或100毫克/千克/天，持续6周）增强了对股骨骨密度（BMD）、骨矿物质含量（BMC）以及其他参与骨重塑的因素的抑制作用。此外，eGr略微降低了由OVX增加的骨转换标志物。因此，可以认为eGr的保护作用可能源于抑制OVX诱导的骨转换增加。总体而言，本研究结果表明，eGr有潜力通过抑制破骨细胞分化和骨质流失来激活骨重塑。
