性激素结合球蛋白对睾酮和雌二醇直接放射免疫测定干扰的研究。

Investigation of sex-hormone binding globulin interference in direct radioimmunoassays for testosterone and estradiol.

作者信息

Masters A M, Hähnel R

机构信息

Endocrine Research Laboratories, King Edward Memorial Hospital for Women, Australia.

出版信息

Clin Chem. 1989 Jun;35(6):979-84.

PMID:2731371

Abstract

The aim of this study was to find reasons for the interference of sex-hormone binding globulin (SHBG) in direct radioimmunoassays (RIAs) for testosterone and estradiol. 125I-labeled testosterone and estradiol were compared with 3H-labeled testosterone and estradiol for their affinity to SHBG. The 3H-labeled steroids bound far more avidly to SHBG than did the corresponding 125I-labeled steroids. The SHBG concentration in the standard matrix of two kits was significant. Finally, 3H-labeled steroids were compared with 125I-labeled steroids for their affinity to the antibody supplied with the kits. The 125I-labeled steroids bound more avidly than did the 3H-labeled steroids to the corresponding antibodies. Direct testosterone and estradiol results would be influenced according to the SHBG in the sample, as observed.

摘要

本研究的目的是找出性激素结合球蛋白（SHBG）干扰睾酮和雌二醇直接放射免疫分析（RIA）的原因。将125I标记的睾酮和雌二醇与3H标记的睾酮和雌二醇对SHBG的亲和力进行比较。3H标记的类固醇与SHBG的结合远比相应的125I标记的类固醇更紧密。两种试剂盒标准基质中的SHBG浓度有显著差异。最后，将3H标记的类固醇与125I标记的类固醇对试剂盒所提供抗体的亲和力进行比较。125I标记的类固醇比3H标记的类固醇与相应抗体的结合更紧密。正如所观察到的，样本中的SHBG会影响直接睾酮和雌二醇的检测结果。