Batycka-Baran Aleksandra, Besgen Petra, Wolf Ronald, Szepietowski Jacek C, Prinz Joerg C
Department of Dermatology, Venereology and Allergology, Wroclaw Medical University, Wroclaw, Poland.
Department of Dermatology and Allergology, Ludwig-Maximilian University, Munich, Germany.
J Photochem Photobiol B. 2016 Aug;161:396-401. doi: 10.1016/j.jphotobiol.2016.05.023. Epub 2016 Jun 1.
Psoriasis is a common, chronic immune-mediated inflammatory disease. The inflammatory process in psoriasis has systemic effects and may influence the development of psoriatic comorbidities. The systemic action of phototherapy in patients with psoriasis has been so far poorly elucidated. We aimed to investigate the expression of genes encoding selected psoriasis-related cytokines in peripheral blood mononuclear cells (PBMCs) isolated from patients with psoriasis before and after treatment with phototherapy. 17 patients with mild to moderate plaque psoriasis were treated with narrow band-UVB (NB-UVB), 8 patients with moderate to severe plaque psoriasis with bath-psoralen-ultraviolet A therapy (PUVA). PBMCs were isolated by Ficoll gradient density centrifugation. Expression of genes encoding TNF-α, IL-17A, IL-6, IL-1 β, INF-γ, and IL-10 in PBMCs of patients with psoriasis before and after phototherapy was analyzed with quantitative RT-PCR. Treatment with NB-UVB therapy led to a significant decrease in IL-17A, TNF-α, and IL-6 mRNA levels in PBMCs (p=0.003; p=0.042; p=0.019, respectively). Following treatment with bath-PUVA therapy, we observed a significant decrease in TNF-α and IL-6 mRNA levels in PBMCs (p=0.031, p=0.035, respectively). Treatment with phototherapy in patients with psoriasis may affect systemic inflammation by downregulation of the expression of genes encoding proinflammatory cytokines in PBMCs, implicated in the development of psoriasis and psoriatic comorbidities.
银屑病是一种常见的慢性免疫介导的炎症性疾病。银屑病中的炎症过程具有全身效应，可能会影响银屑病合并症的发展。迄今为止，光疗对银屑病患者的全身作用尚未得到充分阐明。我们旨在研究从银屑病患者分离的外周血单核细胞（PBMCs）中，在光疗前后编码选定银屑病相关细胞因子的基因表达情况。17例轻度至中度斑块状银屑病患者接受窄谱中波紫外线（NB-UVB）治疗，8例中度至重度斑块状银屑病患者接受补骨脂素紫外线A浴疗法（PUVA）。通过Ficoll梯度密度离心法分离PBMCs。采用定量逆转录-聚合酶链反应（RT-PCR）分析银屑病患者光疗前后PBMCs中编码肿瘤坏死因子-α（TNF-α）、白细胞介素-17A（IL-17A）、白细胞介素-6（IL-6）、白细胞介素-1β（IL-1β）、干扰素-γ（INF-γ）和白细胞介素-10（IL-10）的基因表达。NB-UVB治疗导致PBMCs中IL-17A、TNF-α和IL-6 mRNA水平显著降低（分别为p = 0.003；p = 0.042；p = 0.019）。浴用PUVA治疗后，我们观察到PBMCs中TNF-α和IL-6 mRNA水平显著降低（分别为p = 0.031，p = 0.035）。对银屑病患者进行光疗可能通过下调PBMCs中编码促炎细胞因子的基因表达来影响全身炎症，这些促炎细胞因子与银屑病和银屑病合并症的发展有关。
