Tian Ye, Tian Xin, Han Xu, Chen Yong, Song Cheng-Yang, Zhang Yan-Bin, Tian Da-Li
Department of Thoracic Surgery, The Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning 110032, P.R. China.
Department of Molecular Oncology, Cancer Research Institute, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110032, P.R. China.
Mol Med Rep. 2016 Aug;14(2):1345-50. doi: 10.3892/mmr.2016.5388. Epub 2016 Jun 10.
ATP binding cassette E1 (ABCE1), a member of the family of ATP binding cassette transporters, has initially emerged as an RNase L inhibitor. As a highly conserved protein, it is involved in capsid assembly and translation processes of the human immunodeficiency virus as well as in tumor development and progression. Studies have shown that ABCE1 protein was overexpressed in lung carcinoma tissues and metastatic lymph nodes compared to normal lung tissues. However, little is known about the roles of ABCE1 in lung cancer. The present study investigated the biological effects of vector-mediated ABCE1 overexpression in lung cancer cells in vitro and examined the underlying molecular mechanisms. Overexpression of ABCE1 in the LTEP‑a-2 lung adenocarcinoma cell line was achieved by transfection with a plasmid containing full‑length ABCE1 cDNA. The ectopic expression of ABCE1 was shown to promote the viability and invasive capacity of lung cancer cells, and to in reduce p27 expression. However, overexpression of ABCE1 did not significantly affect the cell cycle distribution. In conclusion, the present study suggested that ABCE1 promotes the growth, invasion and metastasis of lung adenocarcinoma cells and may represent a potential biomarker and therapeutic target for lung cancer.
ATP结合盒E1（ABCE1）是ATP结合盒转运蛋白家族的成员，最初作为核糖核酸酶L抑制剂出现。作为一种高度保守的蛋白质，它参与人类免疫缺陷病毒的衣壳组装和翻译过程以及肿瘤的发生和发展。研究表明，与正常肺组织相比，ABCE1蛋白在肺癌组织和转移淋巴结中过表达。然而，ABCE1在肺癌中的作用鲜为人知。本研究调查了载体介导的ABCE1在肺癌细胞中的过表达的生物学效应，并探讨了其潜在的分子机制。通过转染含有全长ABCE1 cDNA的质粒，在LTEP-a-2肺腺癌细胞系中实现了ABCE1的过表达。结果显示，ABCE1的异位表达促进肺癌细胞的活力和侵袭能力，并降低p27表达。然而，ABCE1的过表达对细胞周期分布没有显著影响。总之，本研究表明ABCE1促进肺腺癌细胞的生长、侵袭和转移，可能是肺癌的潜在生物标志物和治疗靶点。
