Rodríguez-Fernández C, Ruiz-Larrea M F
Department of Biochemistry, Faculty of Science, University of the Basque Country, Bilbao, Spain.
Enzyme. 1989;41(1):27-32. doi: 10.1159/000469047.
A kinetic study of neutral and alkaline triglyceride lipase activities from different liver homogenate fractions is reported. Lipase activities are studied with triolein as substrate and are determined by quantification of the released oleic acid liberated. Heparin-releasable, microsomal and mitochondrial lipase activities are studied as a function of time, protein concentration and substrate concentration. The neutral triglyceride lipase associated with mitochondrial membranes is kinetically different from the alkaline lipase, localized on the plasma membrane, which probably contaminates microsomal and soluble fractions.
本文报道了对来自不同肝脏匀浆组分的中性和碱性甘油三酯脂肪酶活性的动力学研究。以三油酸甘油酯作为底物研究脂肪酶活性，并通过对释放出的油酸进行定量测定来确定活性。研究了肝素可释放的、微粒体和线粒体脂肪酶活性随时间、蛋白质浓度和底物浓度的变化情况。与线粒体膜相关的中性甘油三酯脂肪酶在动力学上与位于质膜上的碱性脂肪酶不同，质膜可能污染了微粒体和可溶组分。
