Ruiz-Larrea M F, Galdiz-Valdovinos B, Rodríguez-Fernández C
Enzyme. 1982;27(3):215-9. doi: 10.1159/000459051.
An assay is described for the determination of lipase activity, using as substrate an emulsion of radioactive triolein with cholic acid as emulsifier. Lipase activity is given by determining radioactive triolein disappearance after incubation with the artificial emulsion. The catalytic activity of this enzyme has been studied with respect to time, protein concentration and substrate concentration. Under the experimental conditions, Michaelis constant value was 14.54 mmol/l and maximal hydrolysis rate 0.31 mumol h-1 mg-1.
本文描述了一种用于测定脂肪酶活性的分析方法，该方法使用以胆酸作为乳化剂的放射性三油酸甘油酯乳剂作为底物。通过测定与人工乳剂孵育后放射性三油酸甘油酯的消失量来确定脂肪酶活性。已经研究了该酶在时间、蛋白质浓度和底物浓度方面的催化活性。在实验条件下，米氏常数值为14.54 mmol/l，最大水解速率为0.31 μmol h-1 mg-1。
