Hietter H, Luu B, Goltzene F, Zachary D, Hoffmann J, Van Dorsselaer A
Laboratoire de chimie organique des substances naturelles, associé au Centre national de la recherche scientifique, Strasbourg, France.
Eur J Biochem. 1989 Jun 1;182(1):77-84. doi: 10.1111/j.1432-1033.1989.tb14802.x.
We have isolated two major 6-kDa peptides from extracts of corpora cardiaca of adult females of Locusta migratoria. These peptides have been characterized by peptide sequencing and liquid secondary-ion mass spectrometry. They are structurally related dimers, one (6278.5 Da) being a homodimer (A-A chains), the other (6280.5 Da) being a heterodimer (A-B chains). A 60% similarity exists between the A and B chains. Both peptides have been chemically synthesized and the synthetic compounds appeared to be identical to the native ones. Polyclonal antibodies raised against each of these peptides demonstrated that they were contained within the secretory granules of the intrinsic cells of the glandular lobes of the corpora cardiaca. The physiological significance of these two peptides is unknown but, using the synthetic peptides, we are currently probing their biological role.
我们从成年雌性飞蝗心侧体提取物中分离出了两种主要的6 kDa肽。这些肽已通过肽测序和液相二次离子质谱进行了表征。它们是结构相关的二聚体，一种（6278.5 Da）是同二聚体（A - A链），另一种（6280.5 Da）是异二聚体（A - B链）。A链和B链之间存在60%的相似性。两种肽均已化学合成，且合成化合物似乎与天然化合物相同。针对这些肽各自产生的多克隆抗体表明，它们存在于心侧体腺叶固有细胞的分泌颗粒中。这两种肽的生理意义尚不清楚，但我们目前正在使用合成肽探究它们的生物学作用。
