Hietter H, Van Dorsselaer A, Green B, Denoroy L, Hoffmann J, Luu B
Laboratoire de Chimie Organiques des Substances Naturelles, associé au Centre National de la Recherche Scientifique, Strasbourg, France.
Eur J Biochem. 1990 Jan 12;187(1):241-7. doi: 10.1111/j.1432-1033.1990.tb15301.x.
Two predominant peptides have been isolated from neurohaemal lobes of corpora cardiaca of 8000 adults of Locusta migratoria. Both peptides have been unambiguously characterized by automated peptide microsequencing and liquid secondary-ion mass spectrometry as a 50-residue peptide (5K peptide) and a 48-residue isologue (5K' peptide). Computer search of sequence data banks did not reveal any significant similarity with other identified proteins. The 5K peptides are remarkably rich in alanine residues (25%) and contain a stretch of five consecutive alanines. This structure suggests that these molecules could correspond to spacer peptides. This assumption is corroborated in the accompanying paper [Lagueux et al. (1990) Eur. J. Biochem. 187, 249-254] on the molecular cloning of the precursor protein which attributes to the 5K peptides a role analogous to that of the C peptides of insulins.
从8000只成年飞蝗的心侧体神经血叶中分离出了两种主要的肽。通过自动肽微测序和液体二次离子质谱法，这两种肽已被明确鉴定为一种50个残基的肽（5K肽）和一种48个残基的同系物（5K'肽）。对序列数据库的计算机搜索未发现与其他已鉴定蛋白质有任何显著相似性。5K肽富含丙氨酸残基（25%），并包含一段连续五个丙氨酸的序列。这种结构表明这些分子可能对应间隔肽。在随附论文[拉格厄克斯等人（1990年）《欧洲生物化学杂志》187卷，249 - 254页]中关于前体蛋白分子克隆的内容证实了这一假设，该论文认为5K肽的作用类似于胰岛素的C肽。
