He Zhiyong, Chen Yuqi, Wang Yafen, Wang Jiaqi, Mo Jing, Fu Boshi, Wang Zijing, Du Yuhao, Zhou Xiang
College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei 430072, P. R. China.
Chem Commun (Camb). 2016 Jun 30;52(55):8545-8. doi: 10.1039/c6cc03098j.
A new DNA building block (d(Tet)U) bearing a tetrazole and allyloxy group at N-phenyl ring linked through an aminopropynyl linker to the 5-position of 2'-deoxyuridine was synthesized. The modified DNA can be lit up via a photoinduced intramolecular tetrazole-alkene cycloaddition reaction, but quenched when the fully-matched double strand is formed. This conspicuous difference in fluorescence could open a door for DNA single nucleotide polymorphism (SNP) typing.
合成了一种新的DNA构建单元（d(Tet)U），其在N-苯环上带有四唑和烯丙氧基，通过氨基丙炔基连接子连接到2'-脱氧尿苷的5位。修饰后的DNA可通过光诱导分子内四唑-烯烃环加成反应发光，但在形成完全匹配的双链时会淬灭。这种荧光上的显著差异可为DNA单核苷酸多态性（SNP）分型打开一扇门。
