Ishii M, Ono T, Suzuki K, Igarashi Y, Kodama T
a Department of Agricultural Chemistry , The University of Tokyo , Bunkyo-ku, Tokyo 113 , Japan.
b Riken , Japan Collection of Microorganisms , Wako-shi, Saitama 351-01 , Japan.
Biosci Biotechnol Biochem. 1994 Jan;58(1):1-4. doi: 10.1271/bbb.58.1.
The electron transport system in Pseudomonas hydrogenothermophila strain TH-1, a thermophilic, aerobic hydrogen-oxidizing bacterium, was studied using a membrane fraction, an ubiquinone-depleted membrane fraction (Q(-) MF), and an ubiqunone-reconstituted membrane fraction (Q(+) MF). From spectrophotometric and TLC analyses, cytochromes b and c, ubiquinone-8 (Q8), and cytochrome o were detected as respiratory components in the membrane fraction of P. hydrogenothermophila. Spectrophotometric analyses also showed that reduction of all the components in the membrane fraction depends on a membrane-bound hydrogenase reaction. In Q(-) MF, reduction of cytochromes b and c depending on membrane-bound hydrogenase reaction could not be observed. However, the reduction was restored in Q(+) MF. From these results, a possible electron transport system in P. hydrogenothermophila strain TH-1 is proposed.
利用膜组分、泛醌耗尽的膜组分（Q(-) MF）和泛醌重构的膜组分（Q(+) MF），对嗜热需氧氢氧化细菌嗜热栖氢假单胞菌菌株TH-1中的电子传递系统进行了研究。通过分光光度法和薄层色谱分析，在嗜热栖氢假单胞菌的膜组分中检测到细胞色素b和c、泛醌-8（Q8）以及细胞色素o作为呼吸成分。分光光度分析还表明，膜组分中所有成分的还原都依赖于膜结合氢化酶反应。在Q(-) MF中，未观察到依赖于膜结合氢化酶反应的细胞色素b和c的还原。然而，在Q(+) MF中这种还原得以恢复。基于这些结果，提出了嗜热栖氢假单胞菌菌株TH-1中可能的电子传递系统。
