Hayashi N R, Ishida T, Yokota A, Kodama T, Igarashi Y
Department of Biotechnology, University of Tokyo, Japan.
Int J Syst Bacteriol. 1999 Apr;49 Pt 2:783-6. doi: 10.1099/00207713-49-2-783.
The taxonomic positions of 'Pseudomonas hydrogenothermophila' strain TH-1 and 'Flavobacterium autothermophilum' strain TH-4 were studied by 16S rDNA sequencing. These organisms are Gram-negative, strictly aerobic, thermophilic, facultatively chemolithoautotrophic hydrogen-oxidizing rods and have a DNA G + C content of 63-65 mol%. The major isoprenoid quinone is ubiquinone-8 and 3-hydroxy decanoic acid (3-OH C10:0) is the major 3-hydroxy cellular fatty acid. A phylogenetic analysis based on 16S rDNA sequences placed strains TH-1T and TH-4 in the beta-subclass of the Proteobacteria. The taxonomic characteristics of these organisms are different from those of previously described aerobic, facultatively chemolithoautotrophic, hydrogen-oxidizing bacteria that belong to the beta-subclass of Proteobacteria. On the basis of the information described above, a new genus and species, Hydrogenophilus thermoluteolus gen. nov., sp. nov., is described to include both strains. The type strain is strain TH-1T (= IFO 14978T).
通过16S rDNA测序研究了“嗜热氢假单胞菌”菌株TH-1和“嗜热自养黄杆菌”菌株TH-4的分类地位。这些微生物为革兰氏阴性、严格需氧、嗜热、兼性化能自养型氢氧化杆菌，DNA的G + C含量为63 - 65mol%。主要类异戊二烯醌为泛醌-8，3-羟基癸酸（3-OH C10:0）是主要的3-羟基细胞脂肪酸。基于16S rDNA序列的系统发育分析将菌株TH-1T和TH-4置于变形菌纲的β亚类中。这些微生物的分类特征与先前描述的属于变形菌纲β亚类的需氧、兼性化能自养型氢氧化细菌不同。基于上述信息，描述了一个新属和新种，嗜热栖热氢杆菌属（Hydrogenophilus thermoluteolus），新属，新种，包括这两个菌株。模式菌株为TH-1T（= IFO 14978T）。
