Choi K W, Han O, Lee H J, Yun Y C, Moon Y H, Kim M, Kuk Y I, Han S U, Guh J O
a Plant Biotechnology Laboratory, Jinro Central Research Institute.
b Institute of Agricultural Science and Technology, Chonnam National University.
Biosci Biotechnol Biochem. 1998;62(3):558-60. doi: 10.1271/bbb.62.558.
In an effort to develop transgenic plants resistant to diphenyl ether herbicides, we introduced the protoporphyrinogen oxidase (EC 1.3.3.4) gene of Bacillus subtilis into tobacco plants. The results from a Northern analysis and leaf disc assay indicate that the expression of the B. subtilis protoporphyrinogen oxidase gene under the cauliflower mosaic virus 35S promoter generated resistance to the diphenyl ether herbicide, oxyfluorfen, in transgenic tobacco plants.
为了培育对二苯醚除草剂具有抗性的转基因植物，我们将枯草芽孢杆菌的原卟啉原氧化酶（EC 1.3.3.4）基因导入烟草植株。Northern分析和叶盘试验结果表明，在花椰菜花叶病毒35S启动子控制下的枯草芽孢杆菌原卟啉原氧化酶基因的表达使转基因烟草植株对二苯醚除草剂乙氧氟草醚产生了抗性。
