• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

原卟啉原氧化酶基因的突变导致水稻出现斑点和卷叶，其过表达导致水稻对除草剂产生抗性。

Mutation of Protoporphyrinogen IX Oxidase Gene Causes Spotted and Rolled Leaf and Its Overexpression Generates Herbicide Resistance in Rice.

机构信息

State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, China.

Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China.

出版信息

Int J Mol Sci. 2022 May 21;23(10):5781. doi: 10.3390/ijms23105781.

DOI:10.3390/ijms23105781
PMID:35628595
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9146718/
Abstract

Protoporphyrinogen IX (Protogen IX) oxidase (PPO) catalyzes the oxidation of Protogen IX to Proto IX. PPO is also the target site for diphenyl ether-type herbicides. In plants, there are two encoding genes, and . To date, no gene or mutant has been characterized in monocotyledonous plants. In this study, we isolated a () mutant in rice (). The spotted leaf phenotype was sensitive to high light intensity and low temperature, but the rolled leaf phenotype was insensitive. We confirmed that the phenotypes were caused by a single nucleotide substitution in the () gene. This gene is constitutively expressed, and its encoded product is localized to the chloroplast. The mutant accumulated excess Proto(gen) IX and reactive oxygen species (ROS), resulting in necrotic lesions. The expressions of 26 genes associated with tetrapyrrole biosynthesis, photosynthesis, ROS accumulation, and rolled leaf were significantly altered in , demonstrating that these expression changes were coincident with the mutant phenotypes. Importantly, -overexpression transgenic plants were resistant to the herbicides oxyfluorfen and acifluorfen under field conditions, while having no distinct influence on plant growth and grain yield. These finding indicate that the gene has the potential to engineer herbicide resistance in rice.

摘要

原卟啉原 IX（Proto IX）氧化酶（PPO）催化原卟啉原 IX 氧化为原卟啉 IX。PPO 也是二苯醚类除草剂的靶标位点。在植物中，有两个编码基因 和 。迄今为止，在单子叶植物中尚未对 基因或突变体进行过特征描述。在这项研究中，我们在水稻（）中分离出一个 （）突变体。该斑点叶表型对高光强和低温敏感，但卷曲叶表型不敏感。我们证实 表型是由 （）基因中的单个核苷酸取代引起的。该基因组成型表达，其编码产物定位于叶绿体。 突变体积累了过量的原卟啉（gen）IX 和活性氧（ROS），导致坏死病变。与四吡咯生物合成、光合作用、ROS 积累和卷曲叶相关的 26 个基因的表达在 中显著改变，表明这些表达变化与突变体表型一致。重要的是，在田间条件下，-过表达转基因植物对除草剂草枯醚和乙氧氟草醚具有抗性，而对植物生长和籽粒产量没有明显影响。这些发现表明， 基因有可能在水稻中工程化抗除草剂。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d32/9146718/ca619eaa9a4f/ijms-23-05781-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d32/9146718/e0c9963242dc/ijms-23-05781-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d32/9146718/0c921070ed4b/ijms-23-05781-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d32/9146718/b20844ba8bc1/ijms-23-05781-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d32/9146718/853d0a39ff7f/ijms-23-05781-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d32/9146718/93f232f7100f/ijms-23-05781-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d32/9146718/c01684758271/ijms-23-05781-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d32/9146718/aa44ad7d540e/ijms-23-05781-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d32/9146718/28b5dd0dd154/ijms-23-05781-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d32/9146718/8f5552958f13/ijms-23-05781-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d32/9146718/3b4b17b6db2a/ijms-23-05781-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d32/9146718/4dda1eb23268/ijms-23-05781-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d32/9146718/bfda23fffdd0/ijms-23-05781-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d32/9146718/ca619eaa9a4f/ijms-23-05781-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d32/9146718/e0c9963242dc/ijms-23-05781-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d32/9146718/0c921070ed4b/ijms-23-05781-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d32/9146718/b20844ba8bc1/ijms-23-05781-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d32/9146718/853d0a39ff7f/ijms-23-05781-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d32/9146718/93f232f7100f/ijms-23-05781-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d32/9146718/c01684758271/ijms-23-05781-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d32/9146718/aa44ad7d540e/ijms-23-05781-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d32/9146718/28b5dd0dd154/ijms-23-05781-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d32/9146718/8f5552958f13/ijms-23-05781-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d32/9146718/3b4b17b6db2a/ijms-23-05781-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d32/9146718/4dda1eb23268/ijms-23-05781-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d32/9146718/bfda23fffdd0/ijms-23-05781-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d32/9146718/ca619eaa9a4f/ijms-23-05781-g013.jpg

相似文献

1
Mutation of Protoporphyrinogen IX Oxidase Gene Causes Spotted and Rolled Leaf and Its Overexpression Generates Herbicide Resistance in Rice.原卟啉原氧化酶基因的突变导致水稻出现斑点和卷叶，其过表达导致水稻对除草剂产生抗性。
Int J Mol Sci. 2022 May 21;23(10):5781. doi: 10.3390/ijms23105781.
2
Toxic tetrapyrrole accumulation in protoporphyrinogen IX oxidase-overexpressing transgenic rice plants.原卟啉原IX氧化酶过表达转基因水稻植株中有毒四吡咯的积累。
Plant Mol Biol. 2008 Jul;67(5):535-46. doi: 10.1007/s11103-008-9338-0. Epub 2008 Apr 24.
3
Origins and structure of chloroplastic and mitochondrial plant protoporphyrinogen oxidases: implications for the evolution of herbicide resistance.质体和线粒体植物原卟啉原氧化酶的起源和结构：对除草剂抗性进化的影响。
Pest Manag Sci. 2018 Oct;74(10):2226-2234. doi: 10.1002/ps.4744. Epub 2017 Nov 22.
4
Overexpression of plastidic protoporphyrinogen IX oxidase leads to resistance to the diphenyl-ether herbicide acifluorfen.质体原卟啉原IX氧化酶的过表达导致对二苯醚除草剂三氟羧草醚产生抗性。
Plant Physiol. 2000 Jan;122(1):75-84. doi: 10.1104/pp.122.1.75.
5
Biochemical and physiological mode of action of tiafenacil, a new protoporphyrinogen IX oxidase-inhibiting herbicide.新型原卟啉原氧化酶抑制剂除草剂噻呋酰胺的生化和生理作用模式。
Pestic Biochem Physiol. 2018 Nov;152:38-44. doi: 10.1016/j.pestbp.2018.08.010. Epub 2018 Aug 24.
6
Inhibition profile of trifludimoxazin towards PPO2 target site mutations.三氟多杀菌素对 PPO2 靶标突变的抑制谱。
Pest Manag Sci. 2023 Feb;79(2):507-519. doi: 10.1002/ps.7216. Epub 2022 Oct 17.
7
Field-Evolved from Palmer Amaranth Confers Pre-emergence Tolerance to PPO-Inhibitors in Rice and .在稻田中，由野茼蒿进化而来的对 PPO 抑制剂具有芽前耐受性。
Genes (Basel). 2022 Jun 10;13(6):1044. doi: 10.3390/genes13061044.
8
Encoding a Specific and Conservative Protein for Photosynthetic Organisms Affects Tetrapyrrole Biosynthesis, Photosynthesis, and Reactive Oxygen Species Metabolism in Rice.编码一种特定且保守的蛋白质对光合生物的影响：在水稻中四吡咯生物合成、光合作用和活性氧代谢。
Int J Mol Sci. 2023 Nov 25;24(23):16762. doi: 10.3390/ijms242316762.
9
Dual plastid targeting of protoporphyrinogen oxidase 2 in Amaranthaceae promotes herbicide tolerance.质体双定位原卟啉原氧化酶 2 促进苋科杂草对除草剂的耐受性。
Plant Physiol. 2024 Apr 30;195(1):713-727. doi: 10.1093/plphys/kiae062.
10
Characterization of HemY-type protoporphyrinogen IX oxidase genes from cyanobacteria and their functioning in transgenic Arabidopsis.从蓝藻中鉴定出 HemY 型原卟啉原 IX 氧化酶基因及其在转基因拟南芥中的功能。
Plant Mol Biol. 2019 Dec;101(6):561-574. doi: 10.1007/s11103-019-00925-8. Epub 2019 Oct 16.

引用本文的文献

1
Insight into the genetics of a novel white-striped leaf in rice.对水稻一种新型白条纹叶的遗传学研究
Front Plant Sci. 2025 Aug 20;16:1622640. doi: 10.3389/fpls.2025.1622640. eCollection 2025.
2
Lesion Mimic Mutant: An Ideal Genetic Material for Deciphering the Balance Between Plant Immunity and Growth.类病变突变体：解析植物免疫与生长平衡的理想遗传材料。
Rice (N Y). 2025 May 13;18(1):34. doi: 10.1186/s12284-025-00789-1.
3
Chlorophyll and Carotenoid Metabolism Varies with Growth Temperatures among Tea Genotypes with Different Leaf Colors in .

本文引用的文献

1
A Single Nucleotide Substitution of GSAM Gene Causes Massive Accumulation of Glutamate 1-Semialdehyde and Yellow Leaf Phenotype in Rice.GSAM基因的单核苷酸替换导致水稻中谷氨酸-1-半醛大量积累和黄叶表型。
Rice (N Y). 2021 Jun 5;14(1):50. doi: 10.1186/s12284-021-00492-x.
2
Disruption of EARLY LESION LEAF 1, encoding a cytochrome P450 monooxygenase, induces ROS accumulation and cell death in rice.编码细胞色素P450单加氧酶的早期病变叶片1发生破坏，会诱导水稻体内活性氧积累和细胞死亡。
Plant J. 2021 Feb;105(4):942-956. doi: 10.1111/tpj.15079. Epub 2020 Dec 20.
3
Rice EARLY SENESCENCE 2, encoding an inositol polyphosphate kinase, is involved in leaf senescence.
叶绿素和类胡萝卜素代谢随不同叶色茶树基因型的生长温度而变化。
Int J Mol Sci. 2024 Oct 7;25(19):10772. doi: 10.3390/ijms251910772.
4
Breeding herbicide-resistant rice using CRISPR-Cas gene editing and other technologies.利用CRISPR-Cas基因编辑技术及其他技术培育抗除草剂水稻。
Plant Commun. 2025 Feb 10;6(2):101172. doi: 10.1016/j.xplc.2024.101172. Epub 2024 Oct 12.
5
An artificially evolved gene for herbicide-resistant rice breeding.抗除草剂水稻培育的人工进化基因。
Proc Natl Acad Sci U S A. 2024 Aug 20;121(34):e2407285121. doi: 10.1073/pnas.2407285121. Epub 2024 Aug 12.
6
Encoding a Specific and Conservative Protein for Photosynthetic Organisms Affects Tetrapyrrole Biosynthesis, Photosynthesis, and Reactive Oxygen Species Metabolism in Rice.编码一种特定且保守的蛋白质对光合生物的影响：在水稻中四吡咯生物合成、光合作用和活性氧代谢。
Int J Mol Sci. 2023 Nov 25;24(23):16762. doi: 10.3390/ijms242316762.
7
Lesion mimic mutant 8 balances disease resistance and growth in rice.类病变突变体8平衡水稻的抗病性与生长。
Front Plant Sci. 2023 Jun 5;14:1189926. doi: 10.3389/fpls.2023.1189926. eCollection 2023.
8
A Locus Controlling Leaf Rolling Degree in Wheat under Drought Stress Identified by Bulked Segregant Analysis.通过混合分组分析法鉴定的干旱胁迫下小麦叶片卷曲度的一个基因座
Plants (Basel). 2022 Aug 9;11(16):2076. doi: 10.3390/plants11162076.
水稻 EARLY SENESCENCE 2，编码肌醇多磷酸激酶，参与叶片衰老。
BMC Plant Biol. 2020 Aug 26;20(1):393. doi: 10.1186/s12870-020-02610-1.
4
A lil3 chlp double mutant with exclusive accumulation of geranylgeranyl chlorophyll displays a lethal phenotype in rice.一个含有 geranylgeranyl chlorophyll 专一性积累的 lil3 chlp 双突变体在水稻中表现出致死表型。
BMC Plant Biol. 2019 Oct 29;19(1):456. doi: 10.1186/s12870-019-2028-z.
5
Chloroplast-Localized Protoporphyrinogen IX Oxidase1 Is Involved in the Mitotic Cell Cycle in Arabidopsis.质体定位的原卟啉原 IX 氧化酶 1 参与拟南芥有丝分裂细胞周期。
Plant Cell Physiol. 2019 Nov 1;60(11):2436-2448. doi: 10.1093/pcp/pcz135.
6
The catalytic subunit of magnesium-protoporphyrin IX monomethyl ester cyclase forms a chloroplast complex to regulate chlorophyll biosynthesis in rice.镁原卟啉 IX 单甲酯环化酶的催化亚基形成叶绿体复合物，以调节水稻中的叶绿素生物合成。
Plant Mol Biol. 2016 Sep;92(1-2):177-91. doi: 10.1007/s11103-016-0513-4. Epub 2016 Aug 11.
7
REL2, A Gene Encoding An Unknown Function Protein which Contains DUF630 and DUF632 Domains Controls Leaf Rolling in Rice.REL2，一个编码一个具有 DUF630 和 DUF632 结构域的未知功能蛋白的基因，控制水稻叶片卷曲。
Rice (N Y). 2016 Dec;9(1):37. doi: 10.1186/s12284-016-0105-6. Epub 2016 Jul 29.
8
Posttranslational Control of ALA Synthesis Includes GluTR Degradation by Clp Protease and Stabilization by GluTR-Binding Protein.δ-氨基-γ-酮戊酸合成的翻译后调控包括Clp蛋白酶介导的谷氨酸-tRNA还原酶降解以及谷氨酸-tRNA还原酶结合蛋白介导的稳定作用。
Plant Physiol. 2016 Apr;170(4):2040-51. doi: 10.1104/pp.15.01945. Epub 2016 Feb 16.
9
PGL, encoding chlorophyllide a oxygenase 1, impacts leaf senescence and indirectly affects grain yield and quality in rice.编码叶绿素酸酯a加氧酶1的PGL影响水稻叶片衰老，并间接影响水稻的产量和品质。
J Exp Bot. 2016 Mar;67(5):1297-310. doi: 10.1093/jxb/erv529. Epub 2015 Dec 25.
10
Phytochrome B Mediates the Regulation of Chlorophyll Biosynthesis through Transcriptional Regulation of ChlH and GUN4 in Rice Seedlings.光敏色素B通过转录调控水稻幼苗中的ChlH和GUN4介导叶绿素生物合成的调节。
PLoS One. 2015 Aug 13;10(8):e0135408. doi: 10.1371/journal.pone.0135408. eCollection 2015.