Spooner B S, Bassett K E, Spooner B S
Division of Biology, Kansas State University, Manhattan 66506.
Dev Biol. 1989 Jun;133(2):569-75. doi: 10.1016/0012-1606(89)90059-6.
Embryonic mouse submandibular salivary gland rudiments undergo morphogenesis in organ culture, characterized by extensive epithelial growth and expansion and repetitive branching activity. Tunicamycin, at a concentration of 25 ng/ml culture medium, decreases the degree of net protein accumulation by 83% and the degree of epithelial expansion by 70% compared to controls, over a 48-hr culture tenure. These decreases correlate with reduced incorporation of [3H]thymidine into DNA. Nevertheless, epithelial branching activity is uncompromised, undergoing an approximately 10-fold increase in lobe numbers, in both controls and tunicamycin-treated rudiments, during the same 48-hr period. The effect is most striking during the 24- to 48-hr culture interval, when controls and tunicamycin-treated rudiments each triple their lobe numbers and controls approximately double epithelial area, while tunicamycin virtually stops all epithelial expansion.
胚胎期小鼠下颌下唾液腺原基在器官培养中发生形态发生，其特征为广泛的上皮生长与扩张以及重复性分支活动。与对照组相比，在浓度为25 ng/ml培养基的衣霉素作用下，经过48小时的培养期，净蛋白积累程度降低了83%，上皮扩张程度降低了70%。这些降低与[3H]胸苷掺入DNA的减少相关。然而，上皮分支活动并未受到影响，在相同的48小时期间，对照组和经衣霉素处理的原基中的叶数量均增加了约10倍。在24至48小时的培养间隔内，这种效应最为显著，此时对照组和经衣霉素处理的原基的叶数量均增加两倍，对照组的上皮面积增加约一倍，而衣霉素实际上使所有上皮扩张停止。
