Makyio Hisayoshi, Shimabukuro Junpei, Suzuki Tatsuya, Imamura Akihiro, Ishida Hideharu, Kiso Makoto, Ando Hiromune, Kato Ryuichi
Structural Biology Research Center, Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK), 1-1 Oho, Tsukuba, Ibaraki, 305-0801, Japan.
Department of Applied Bioorganic Chemistry, Gifu University, 1-1 Yanagido, Gifu-shi, Gifu 501-1193, Japan; Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida Ushinomiya-cho, Sakyo-ku, Kyoto 606-8501, Japan.
Biochem Biophys Res Commun. 2016 Aug 26;477(3):477-82. doi: 10.1016/j.bbrc.2016.06.069. Epub 2016 Jun 16.
The crystal structure of AOL (a fucose-specific lectin of Aspergillus oryzae) has been solved by SAD (single-wavelength anomalous diffraction) and MAD (multi-wavelength anomalous diffraction) phasing of seleno-fucosides. The overall structure is a six-bladed β-propeller similar to that of other fucose-specific lectins. The fucose moieties of the seleno-fucosides are located in six fucose-binding sites. Although the Arg and Glu/Gln residues bound to the fucose moiety are common to all fucose-binding sites, the amino-acid residues involved in fucose binding at each site are not identical. The varying peak heights of the seleniums in the electron density map suggest that each fucose-binding site has a different carbohydrate binding affinity.
米曲霉岩藻糖特异性凝集素（AOL）的晶体结构已通过硒代岩藻糖苷的单波长异常衍射（SAD）和多波长异常衍射（MAD）相位解析得到。其整体结构是一个六叶β-螺旋桨，与其他岩藻糖特异性凝集素的结构相似。硒代岩藻糖苷的岩藻糖部分位于六个岩藻糖结合位点。虽然与岩藻糖部分结合的精氨酸和谷氨酸/谷氨酰胺残基在所有岩藻糖结合位点都很常见，但每个位点参与岩藻糖结合的氨基酸残基并不相同。电子密度图中硒的峰值高度不同，表明每个岩藻糖结合位点具有不同的碳水化合物结合亲和力。
