Barg P E, Wahrman M Z, Talansky B E, Gordon J W
J Exp Zool. 1986 Mar;237(3):365-74. doi: 10.1002/jez.1402370309.
We have devised a procedure for mechanically inserting intact, acrosome reacted spermatozoa under the mouse zona pellucida, and have examined the ability of sperm so inserted to fertilize the mouse oocyte. Sperm immobilized by a variety of different methods are unable to fertilize the egg, despite the fact that electron microscopy confirms that they are acrosome reacted. Control experiments show that the oocytes are capable of being fertilized by motile sperm after the microinjection procedure, and that the immobilized sperm are able to form male pronuclei after injection directly into the ctyoplasm. These results indicate that in addition to its importance for penetration of egg investments, sperm motility is required for fusion of the gametes. Alternatively, the findings suggest that the enzymatic machinery required for sperm motility is very similar to that utilized for gamete fusion, and that destruction of one is likely to lead to inactivation of the other.
我们设计了一种将完整的、已发生顶体反应的精子机械插入小鼠透明带下方的方法，并研究了如此插入的精子使小鼠卵母细胞受精的能力。尽管电子显微镜证实通过多种不同方法固定的精子已发生顶体反应，但它们无法使卵子受精。对照实验表明，显微注射后，卵母细胞能够被活动精子受精，并且固定的精子直接注入细胞质后能够形成雄原核。这些结果表明，除了对穿透卵子保护层很重要外，配子融合还需要精子运动。或者，这些发现表明，精子运动所需的酶机制与配子融合所利用的酶机制非常相似，破坏其中一个可能会导致另一个失活。
