Hahn Thomas, Klemm Andrea, Ziesse Patrick, Harms Karsten, Wach Wolfgang, Rupp Steffen, Hirth Thomas, Zibek Susanne
Nat Prod Commun. 2016 May;11(5):689-92.
The optimization and scale-up of inulin extraction from Taraxacum kok-saghyz Rodin was successfully performed. Evaluating solubility investigations, the extraction temperature was fixed at 85 degrees C. The inulin stability regarding degradation or hydrolysis could be confirmed by extraction in the presence of model inulin. Confirming stability at the given conditions the isolation procedure was transferred from a 1 L- to a 1 m3-reactor. The Reynolds number was selected as the relevant dimensionless number that has to remain constant in both scales. The stirrer speed in the large scale was adjusted to 3.25 rpm regarding a 300 rpm stirrer speed in the 1 L-scale and relevant physical and process engineering parameters. Assumptions were confirmed by approximately homologous extraction kinetics in both scales. Since T. kok-saghyz is in the focus of research due to its rubber content side-product isolation from residual biomass it is of great economic interest. Inulin is one of these additional side-products that can be isolated in high quantity (- 35% of dry mass) and with a high average degree of polymerization (15.5) in large scale with a purity of 77%.
成功实现了从橡胶草（Taraxacum kok-saghyz Rodin）中提取菊粉的工艺优化及放大。通过溶解度研究，将提取温度设定为85摄氏度。在模型菊粉存在的情况下进行提取，可证实菊粉在降解或水解方面的稳定性。在给定条件下确认稳定性后，将分离工艺从1升反应器扩大至1立方米反应器。选择雷诺数作为相关无量纲数，该数值在两个规模下都必须保持恒定。根据1升规模下300转/分钟的搅拌速度以及相关物理和过程工程参数，将大规模搅拌速度调整为3.25转/分钟。两个规模下近似同源的提取动力学证实了相关假设。由于橡胶草因其橡胶含量而成为研究重点，从残余生物质中分离副产物具有重大经济意义。菊粉是这些可大量分离（占干重约35%）的副产物之一，大规模生产时平均聚合度高（15.5），纯度达77%。
