Guangxi Key Laboratory for High-Quality Formation and Utilization of Dao-di Herbs, Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China.
Institute of Natural Resources and Ecology, Heilongjiang Academy of Sciences, Harbin 150040, China.
Molecules. 2022 May 31;27(11):3548. doi: 10.3390/molecules27113548.
The roots of Rodin (TKS) are well-known and valued for their rubber-producing ability. Therefore, research on the analysis and detection of metabolites from the roots of TKS have been reported in previous studies. However, all of these studies have the shortcoming of focusing on only the rubber of TKS, without profiling the other metabolites in a systematic and comprehensive way. Here, the primary and secondary metabolites from the leaves of TKS were investigated using UPLC-ESI-MS/MS, and a total of 229 metabolites were characterized. Carboxylic acid derivatives, fatty acyls, phenols, and organooxygen compounds were found to be the major metabolites of TKS. The transcriptome data indicated that ribosomal, glycolysis/gluconeogenesis, phenylpropanoid biosynthesis, and linoleic acid metabolism genes were significantly differentially expressed. This study is the first to report the differences in the metabolic and transcriptome profiles of TKS leaves under exogenous ethephon spray, which improves our understanding of the main metabolites and their molecular mechanisms in TKS leaves.
三叶橡胶树（TKS）的根因其产胶能力而广为人知且备受重视。因此，先前已有研究报道了对 TKS 根代谢产物的分析和检测。然而，所有这些研究都存在一个缺点，即仅关注 TKS 的橡胶，而没有以系统和全面的方式对其他代谢物进行分析。本研究采用 UPLC-ESI-MS/MS 对三叶橡胶树的叶片中的初生和次生代谢产物进行了研究，共鉴定出 229 种代谢产物。发现羧酸衍生物、脂肪酸、酚类和含氧化合物是 TKS 的主要代谢产物。转录组数据表明，核糖体、糖酵解/糖异生、苯丙烷生物合成和亚油酸代谢基因的表达存在显著差异。本研究首次报道了外源乙烯利喷雾处理下三叶橡胶树叶片代谢组和转录组图谱的差异，这有助于我们了解三叶橡胶树叶片中的主要代谢产物及其分子机制。
