Miyagi T, Kubonishi I, Ohtsuki Y, Ohyashiki J H, Toyama K, Miyoshi I
Department of Medicine, Kochi Medical School, Japan.
Int J Cancer. 1989 Jun 15;43(6):1149-54. doi: 10.1002/ijc.2910430631.
A new Philadelphia chromosome (Ph1-positive) acute lymphoblastic leukemia (ALL) cell line was established in nude mice by direct and serial transplantation of peripheral blood leukemia cells from an adult patient. Although the patient's leukemia cells did not grow in vitro, they were successfully transplanted for 8 serial passages, giving rise to progressive growth of tumors with frequent involvement of lymph nodes, liver, spleen, bone marrow, and meninges. The tumor cells could also be passaged in an ascites form. This in vivo cell line, designated PALL-I, retained the Ph1 chromosome, t(9;22) (q34;q11), and pre-B-cell phenotype (SmIg-, CpIg-, CD10+, CD19+, OKIaI+, and CD38+), like the original leukemia cells. Molecular genetic analysis of PALL-I cells revealed neither bcr rearrangement nor 8.5-kb abI-related mRNA that is characteristically seen in Ph1-positive chronic myelogenous leukemia (CML). Thus, the PALL-I cell line is genetically distinct from CML. It may provide a useful model for an understanding of the cellular and molecular biology of Ph1-positive ALL without classical bcr rearrangement.
通过直接和连续移植一名成年患者的外周血白血病细胞，在裸鼠中建立了一种新的费城染色体（Ph1阳性）急性淋巴细胞白血病（ALL）细胞系。尽管患者的白血病细胞在体外不能生长，但它们成功地进行了8次连续传代移植，导致肿瘤逐渐生长，常累及淋巴结、肝脏、脾脏、骨髓和脑膜。肿瘤细胞也可以以腹水形式传代。这种体内细胞系命名为PALL-I，像原始白血病细胞一样，保留了Ph1染色体、t(9;22)(q34;q11)以及前B细胞表型（SmIg-、CpIg-、CD10+、CD19+、OKIaI+和CD38+）。对PALL-I细胞的分子遗传学分析既未发现bcr重排，也未发现Ph1阳性慢性粒细胞白血病（CML）中特有的8.5 kb abI相关mRNA。因此，PALL-I细胞系在遗传学上与CML不同。它可能为理解无经典bcr重排的Ph1阳性ALL的细胞和分子生物学提供一个有用的模型。
