Miyagi T, Ohyashiki J, Yamato K, Koeffler H P, Miyoshi I
Department of Medicine, Kochi Medical School, Japan.
Int J Cancer. 1993 Feb 1;53(3):457-62. doi: 10.1002/ijc.2910530318.
We have established 2 Philadelphia chromosome (Ph1)-positive acute lymphoblastic leukemia (ALL) cell lines, designated PALL-1 and PALL-2, from distinct adult Ph1-positive ALL patients. PALL-1 was established in nude mice, and PALL-2 was established in culture. Both retained the Ph1 chromosome and expressed the ALL type bcr/abl chimeric mRNA containing the junction of the first exon of BCR gene (e1) and second exon of c-abl gene (a2). PALL-1 and PALL-2 expressed CD34 surface antigen which is characteristic of early hematopoietic progenitor cells. PALL-2 expressed antigens for both pre-B and early myeloid cells and had rearrangements of both the heavy chain of immunoglobulin gene and the beta chain of T-cell-receptor gene. Both PALL-1 and PALL-2 expressed detectable levels of p53 gene RNA. Polymerase-chain-reaction-single-strand conformation polymorphism (PCR-SSCP) analysis of the p53 gene showed a normal pattern of mobility in both cell lines. Taken together, the 2 cell lines had features of Ph1-positive ALL: (i) hematopoietic progenitor cells with pre-B-cell phenotype and, (ii) activation of e1-a2 type bcr/abl oncogene without alterations of p53 gene. These unique lines should provide a valuable tool for studying the pathogenesis of Ph1-positive ALL.
我们从不同的成年费城染色体（Ph1）阳性急性淋巴细胞白血病（ALL）患者中建立了2个Ph1阳性ALL细胞系，分别命名为PALL-1和PALL-2。PALL-1是在裸鼠中建立的，PALL-2是在培养物中建立的。二者均保留了Ph1染色体，并表达包含BCR基因第一外显子（e1）与c-abl基因第二外显子（a2）连接处的ALL型bcr/abl嵌合mRNA。PALL-1和PALL-2表达早期造血祖细胞所特有的CD34表面抗原。PALL-2表达前B细胞和早期髓样细胞的抗原，并且免疫球蛋白基因重链和T细胞受体基因β链均发生重排。PALL-1和PALL-2均表达可检测水平的p53基因RNA。对p53基因进行聚合酶链反应-单链构象多态性（PCR-SSCP）分析显示，在这两个细胞系中均呈现正常的迁移模式。综上所述，这两个细胞系具有Ph1阳性ALL的特征：（i）具有前B细胞表型的造血祖细胞，以及（ii）e1-a2型bcr/abl癌基因激活而p53基因无改变。这些独特的细胞系应为研究Ph1阳性ALL的发病机制提供有价值的工具。
