Okada Hiroyuki, Miyazawa Kohtaro, Imamura Morikazu, Iwamaru Yoshifumi, Masujin Kentaro, Matsuura Yuichi, Yokoyama Takashi
National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki 305-0856, Japan.
J Vet Med Sci. 2016 Nov 1;78(10):1619-1624. doi: 10.1292/jvms.16-0259. Epub 2016 Jun 20.
Two Cheviot ewes homozygous for the ALRQ (ALRQ) prion protein (PrP) genotype were exposed intracerebrally to brain pools prepared using four field cases of atypical scrapie from the United Kingdom. Animals were clinically normal until the end of the experiment, when they were culled 7 years post-inoculation. Limited accumulation of disease-associated PrP (PrP) was observed in the cerebellar molecular layer by immunohistochemistry, but not by western blot or enzyme-linked immunosorbent assay. In addition, PrP was partially localized in astrocytes and microglia, suggesting that these cells have a role in PrP processing, degradation or both. Our results indicate that atypical scrapie is transmissible to ALRQ sheep, but these animals act as clinically silent carriers with long incubation times.
两只对ALRQ（ALRQ）朊病毒蛋白（PrP）基因型纯合的切维厄特母羊经脑内接种了使用来自英国的4例非典型羊瘙痒病野外病例制备的脑匀浆。在实验结束前动物临床正常，接种后7年将它们扑杀。通过免疫组织化学在小脑分子层观察到了疾病相关PrP（PrP）的有限积累，但在蛋白质印迹或酶联免疫吸附测定中未观察到。此外，PrP部分定位于星形胶质细胞和小胶质细胞，表明这些细胞在PrP加工、降解或两者中发挥作用。我们的结果表明，非典型羊瘙痒病可传播给ALRQ绵羊，但这些动物作为临床无症状携带者，潜伏期很长。
