Burres N S, Cass C E
McEachern Laboratory, University of Alberta, Edmonton, Canada.
In Vitro Cell Dev Biol. 1989 May;25(5):419-23. doi: 10.1007/BF02624626.
During methotrexate-induced differentiation of cultured human choriocarcinoma (BeWo) cells, proliferation is inhibited, morphologic and biochemical changes occur, and giant, often multinucleated, cells form. We have used the increase in cell volume as a marker of the mature syncytiotrophoblastlike phenotype. Uninduced and differentiated BeWo cells are not spherical, and theoretical considerations suggested that deviations in shape could result in significant errors in Coulter volume. To determine if the values obtained by electrical pulse sizing reflected the actual mass of BeWo cells, we have evaluated the relationship between Coulter volumes and intracellular water volumes obtained using a shape-independent estimate for eight cell types. A close correlation (r2 = 0.97) was found, indicating that cell volume changes in populations of irregularly shaped cells can be accurately measured using a Coulter instrument.
在甲氨蝶呤诱导培养的人绒毛膜癌（BeWo）细胞分化过程中，细胞增殖受到抑制，形态和生化发生改变，形成巨大的、常为多核的细胞。我们将细胞体积增加作为成熟合体滋养层样表型的一个标志。未诱导和已分化的BeWo细胞不是球形的，理论上认为形状偏差可能导致库尔特体积出现显著误差。为了确定通过电脉冲测量得到的值是否反映了BeWo细胞的实际质量，我们评估了库尔特体积与使用形状无关估计法得到的八种细胞类型的细胞内水体积之间的关系。结果发现二者密切相关（r2 = 0.97），表明使用库尔特仪器能够准确测量不规则形状细胞群体中的细胞体积变化。
