Gregg E C, Steidley K D
Biophys J. 1965 Jul;5(4):393-405. doi: 10.1016/S0006-3495(65)86724-8.
A recently developed method of determining the number and size of particles suspended in a conducting solution is to pump the suspension through a small orifice having an immersed electrode on each side to supply electrical current. The current changes due to the passage of particles of resistivity different from that of the solution. Theoretical expressions are developed which relate the current change caused by such particles to their volume and shape. It is found that most biological cells may be treated as dielectric particles whose capacitive effects are negligible. Electrolytic tank measurements on models confirm the theoretical development, and electric field plots of model orifices are used to predict the observed pulse shapes. An equivalent circuit of the orifice-electrode system is analyzed and shows that the current pulse may be made conductivity-independent when observed with a zero input impedance amplifier.
一种最近开发的用于确定悬浮在导电溶液中的颗粒数量和大小的方法是，将悬浮液泵入一个小孔，该小孔两侧各有一个浸入式电极以提供电流。由于电阻率与溶液不同的颗粒通过，电流会发生变化。推导出了理论表达式，将此类颗粒引起的电流变化与其体积和形状联系起来。发现大多数生物细胞可被视为介电颗粒，其电容效应可忽略不计。对模型进行的电解槽测量证实了理论推导，并且使用模型小孔的电场图来预测观察到的脉冲形状。对小孔 - 电极系统的等效电路进行了分析，结果表明，当使用零输入阻抗放大器进行观察时，电流脉冲可以与电导率无关。
