Shah Mickey, George Richard L, Evancho-Chapman M Michelle, Zhang Ge
a Integrated Bioscience Program , The University of Akron , Akron , OH , USA.
b Department of Biomedical Engineering , The University of Akron , Akron , OH , USA.
Organogenesis. 2016 Jul 2;12(3):119-127. doi: 10.1080/15476278.2016.1197461. Epub 2016 Jun 20.
Dedifferentiated fat cells show great promises as a novel cell source for stem cell research. It has many advantages when used for cell-based therapeutics including abundance, pluripotency, and safety. However, there are many obstacles researchers need to overcome to make the next big move in DFAT cells research. In this review, we summarize the current main challenges in DFAT cells research including cell culture purity, phenotypic properties, and dedifferentiation mechanisms. The common methods to produce DFAT cells as well as the cell purity issue during DFAT cell production have been introduced. Current approaches to improve DFAT cell purity have been discussed. The phenotypic profile of DFAT cells have been listed and compared with other stem cells. Further studies on elucidating the underlying dedifferentiation mechanisms will dramatically advance DFAT cell research.
去分化脂肪细胞作为干细胞研究的一种新型细胞来源展现出了巨大的前景。当用于基于细胞的治疗时，它具有诸多优势，包括数量丰富、多能性和安全性。然而，研究人员在去分化脂肪细胞研究中要取得下一步重大进展还需要克服许多障碍。在这篇综述中，我们总结了当前去分化脂肪细胞研究中的主要挑战，包括细胞培养纯度、表型特性和去分化机制。介绍了产生去分化脂肪细胞的常用方法以及去分化脂肪细胞生产过程中的细胞纯度问题。讨论了当前提高去分化脂肪细胞纯度的方法。列出了去分化脂肪细胞的表型特征并与其他干细胞进行了比较。进一步阐明潜在去分化机制的研究将极大地推动去分化脂肪细胞研究。
