Rahman N M A, Fu H, Qiao H, Jin S, Bai H, Zhang W, Jiang F W, Liang G, Sun S, Gong Y, Jiang F F, Xiong Y, Wu Y
Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, China.
Fisheries Research Center, Animal Research Corporation of the Ministry of Animal Resources, Khartoum, Sudan.
Genet Mol Res. 2016 May 25;15(2):gmr7950. doi: 10.4238/gmr.15027950.
Feminization-1 homolog b (Fem1b) is one of the genes essential for male development and play central roles in sex determination of Caenorhabditis elegans. In this study, we cloned and characterized the full-length Fem1b cDNA from the freshwater prawn Macrobrachium nipponense (MnFem1b) in different tissues and at different developmental stages. Real-time quantitative reverse polymerase chain reaction (RT-qPCR) showed that the MnFem1b gene was expressed in all investigated tissues, with the highest expression level found in the testes. The results revealed that the MnFem1b gene might play roles in aspects of development of the male prawn phenotype. The RT-qPCR also revealed that MnFem1b mRNA expression was significantly increased at 10 days after metamorphosis. The expression levels in all investigated tissues showed a certain degree of sexually dimorphism, the expression levels in males were significantly higher than those in females (P < 0.05). Notably, the highest expression of MnFem1b was found in the testes. The expression of MnFem1b in different tissues indicates that it plays multiple biological functions in M. nipponense.
雌性化-1同源物b（Fem1b）是雄性发育所必需的基因之一，在秀丽隐杆线虫的性别决定中起核心作用。在本研究中，我们克隆并鉴定了日本沼虾（MnFem1b）不同组织和不同发育阶段的全长Fem1b cDNA。实时定量逆转录聚合酶链反应（RT-qPCR）表明，MnFem1b基因在所有检测组织中均有表达，在睾丸中表达水平最高。结果表明，MnFem1b基因可能在雄性沼虾表型发育方面发挥作用。RT-qPCR还显示，MnFem1b mRNA表达在变态后10天显著增加。所有检测组织中的表达水平呈现出一定程度的两性差异，雄性中的表达水平显著高于雌性（P < 0.05）。值得注意的是，MnFem1b在睾丸中的表达最高。MnFem1b在不同组织中的表达表明它在日本沼虾中发挥多种生物学功能。
