McKay I, Coia J E, Poxton I R
University Department of Bacteriology, Royal Infirmary, Glasgow.
J Clin Pathol. 1989 May;42(5):511-5. doi: 10.1136/jcp.42.5.511.
In an outbreak of diarrhoeal disease in an orthopaedic ward Clostridium difficile was isolated from all six patients with diarrhoea. Attempts were made to type these isolates by means of antibiogram, detection of pre-formed enzymes, analysis of surface proteins by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting, and plasmid profile analysis. This showed that a single strain (type E) indistinguishable by the four distinct methods of typing, was isolated from all six patients at some time during their episodes of diarrhoea. Relapse was caused by the acquisition of a new strain in two patients, and by re-emergence or reacquisition of the original strain in two patients. The immunochemical method was the most sensitive and discriminatory of the typing strategies adopted.
在一家骨科病房发生的腹泻病暴发中，从所有6例腹泻患者中分离出了艰难梭菌。尝试通过抗菌谱、检测预先形成的酶、用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和免疫印迹法分析表面蛋白以及质粒图谱分析对这些分离株进行分型。结果显示，从所有6例患者腹泻发作期间的某个时间分离出的单一菌株（E型），用四种不同的分型方法无法区分。两名患者的复发是由于获得了一种新菌株，另外两名患者的复发是由于原菌株的再次出现或重新获得。免疫化学方法是所采用的分型策略中最敏感和最具鉴别力的。
