Wolfhagen M J, Fluit A C, Torensma R, Jansze M, Kuypers A F, Verhage E A, Verhoef J
Eijkman-Winkler Institute for Medical and Clinical Microbiology, University Hospital Utrecht, The Netherlands.
J Clin Microbiol. 1993 Aug;31(8):2208-11. doi: 10.1128/jcm.31.8.2208-2211.1993.
A simple discriminative typing method for Clostridium difficile has been developed. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins and restriction enzyme analysis are relatively simple techniques but are difficult to evaluate, especially the restriction enzyme analysis. Immunoblotting and restriction fragment length polymorphism typing facilitate simple discrimination of patterns.
已开发出一种用于艰难梭菌的简单鉴别分型方法。全细胞蛋白的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和限制性内切酶分析是相对简单的技术，但难以评估，尤其是限制性内切酶分析。免疫印迹和限制性片段长度多态性分型有助于简单地区分不同模式。
