Lai P S, Ngeow Y F, Puthucheary S D, Wang C W
J Clin Microbiol. 1983 Jan;17(1):1-6. doi: 10.1128/jcm.17.1.1-6.1983.
Two methods of bacteriocin susceptibility typing for Serratia marcescens were compared. A total of 80 epidemiologically unrelated isolates from patients in a single hospital were typed by the cross-streaking method and the mitomycin C-induced (spotting) method. The cross-streaking method was found to be more discriminatory than the spotting method. Using the cross-streaking method, it was possible to differentiate 50 bacteriocin groups out of the 80 isolates, whereas only 31 groups could be obtained with the spotting method. The reproducibility and percentage typability of the cross-streaking method (82.5 and 93.75%, respectively) were found to be as good as, if not better than, those of the spotting method (78.75 and 90.0%, respectively). Other factors, such as lower economic cost, technical simplicity, and the relative ease in the scoring of results, indicate a preference for the cross-streaking method. The findings of this study support the choice of the cross-streaking method for the bacteriocin typing of S. marcescens in epidemiological studies.
比较了两种用于粘质沙雷氏菌的细菌素敏感性分型方法。采用交叉划线法和丝裂霉素C诱导（点样）法，对一家医院中80株来自流行病学上无关联患者的菌株进行了分型。结果发现，交叉划线法比点样法更具鉴别力。使用交叉划线法，在80株菌株中可区分出50个细菌素组，而点样法只能得到31个组。交叉划线法的重复性和分型率（分别为82.5%和93.75%）即使不比点样法（分别为78.75%和90.0%）更好，也与之相当。其他因素，如较低的经济成本、技术简单性以及结果评分相对容易，表明更倾向于交叉划线法。本研究结果支持在流行病学研究中选择交叉划线法对粘质沙雷氏菌进行细菌素分型。
