Mori Nobuhito, Morimoto Yuya, Takeuchi Shoji
Center for International Research on Integrative Biomedical Systems (CIBiS), Institute of Industrial Science (IIS), The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan.
Center for International Research on Integrative Biomedical Systems (CIBiS), Institute of Industrial Science (IIS), The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan; Takeuchi Biohybrid Innovation Project, Exploratory Research for Advanced Technology (ERATO), Japan Science and Technology (JST), Komaba Open Laboratory (KOL) Room M202, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
J Biosci Bioeng. 2016 Dec;122(6):753-757. doi: 10.1016/j.jbiosc.2016.05.011. Epub 2016 Jun 18.
Vessel-like channels fabricated by embedding sacrificial structures in three-dimensional (3D) cellular constructs and then removing the sacrificial structures have been proposed as a means of providing nutrition to the cells. Alginate gel fibers have been used in the design of such channels owing to their flexibility. However, these channels are closed during culture due to extensive shrinkage of the hydrogel structures when they contain certain cell types such as fibroblasts. Here, we describe a method for fabricating vessel-like channels supported by semi-permeable poly-l-lysine-alginate membrane tubes (PLL-tubes) in a collagen gel. PLL-coated alginate gel fibers were embedded in collagen gel and the inner alginate gel was removed. We were able to form channels in various designs-including branched structures-owing to the flexibility of the alginate gel fibers. Moreover, channels supported by PLL-tubes remained open without shrinkage of the collagen gel containing fibroblasts. These results demonstrate that 3D cellular constructs can be fabricated for culturing cells that would normally induce shrinkage of hydrogel structures.
通过在三维（3D）细胞构建体中嵌入牺牲结构然后去除这些牺牲结构而制造的血管样通道，已被提议作为向细胞提供营养的一种手段。由于其柔韧性，藻酸盐凝胶纤维已被用于此类通道的设计。然而，当这些通道包含某些细胞类型（如成纤维细胞）时，由于水凝胶结构的广泛收缩，它们在培养过程中会封闭。在此，我们描述了一种在胶原凝胶中制造由半透性聚-L-赖氨酸-藻酸盐膜管（PLL管）支撑的血管样通道的方法。将PLL包被的藻酸盐凝胶纤维嵌入胶原凝胶中，并去除内部的藻酸盐凝胶。由于藻酸盐凝胶纤维的柔韧性，我们能够形成各种设计的通道，包括分支结构。此外，由PLL管支撑的通道保持开放，含有成纤维细胞的胶原凝胶不会收缩。这些结果表明，可以制造3D细胞构建体来培养通常会导致水凝胶结构收缩的细胞。
