一种用于体外评估51Cr标记的多形核白细胞趋化性的新的简化单滤器测定法。

A new simplified single-filter assay for 'in vitro' evaluation of chemotaxis of 51Cr-labeled polymorphonuclear leukocytes.

作者信息

Capsoni F, Minonzio F, Ongari A M, Zanussi C

机构信息

Istituto di Clinica Medica I, University of Milan, Ospedale Policlinico, Italy.

出版信息

J Immunol Methods. 1989 Jun 2;120(1):125-31. doi: 10.1016/0022-1759(89)90297-4.

DOI:10.1016/0022-1759(89)90297-4

PMID:2732473

Abstract

A new simplified radioassay for measuring polymorphonuclear leukocyte (PMN) chemotaxis is proposed using 51Cr-labeled cells and a single-filter system. The technique offers all the advantages described for the double-filter radioassay and permits a reproducible measurement of random locomotion, chemokinesis and chemotaxis. Moreover the single-filter radioassay utilizes commercially available and disposable chambers gathered in a multichamber apparatus; this makes the method very easy to learn and rapid to perform.

摘要

本文提出了一种新的简化放射测定法，用于测量多形核白细胞（PMN）的趋化性，该方法使用51Cr标记的细胞和单滤膜系统。该技术具有双滤膜放射测定法所描述的所有优点，并且可以对随机运动、化学增活作用和趋化性进行可重复测量。此外，单滤膜放射测定法使用市售的一次性小室，这些小室集中在一个多小室装置中；这使得该方法非常易于学习且执行迅速。