Kondo Takahisa, Shintani Satoshi, Maeda Kengo, Hayashi Mutsuharu, Inden Yasuya, Numaguchi Yasushi, Sugiura Kaichiro, Morita Yasuhiro, Kitamura Tomoya, Kamiya Haruo, Sone Takahito, Ohno Miyoshi, Murohara Toyoaki
Department of Cardiology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Department of Cardiology, Japanese Red Cross Nagoya Daiichi Hospital, Japan.
Heart Asia. 2010 Jul 11;2(1):20-3. doi: 10.1136/ha.2009.001644. eCollection 2010.
Circulating CD34(+)CD133(+) cells are one of the main sources of circulating endothelial progenitor cells (EPCs). Age is inversely related to the number and function of CD34(+)CD133(+) progenitor cells in stable coronary artery disease (CAD), but the relationship remains unclear in acute myocardial infarction (AMI). The authors aimed to clarify how ageing affects the number and function of mobilised CD34(+)CD133(+) progenitor cells in AMI.
Circulating CD34(+)CD133(+) progenitor cells were measured by flow cytometry. Measurements were made at admission for CAD, or on day 7 after the onset of AMI. In stable CAD (n=131), circulating CD34(+)CD133(+) cells decreased with age (r=-0.344, p<0.0001). In AMI, circulating CD34(+)CD133(+) cells did not correlate with age (n=50), and multivariate analysis revealed that the decreased number of circulating CD34(+)CD133(+) cells was associated with male sex and higher peak creatinine kinase. The ability to give rise to functional EPCs, which show good migratory and tube-forming capabilities, deteriorated among stable CAD subjects (n=10) compared with AMI subjects (N=6).
In stable CAD, the number and function of circulating CD34(+)CD133(+) progenitor cells decreased with age, whereas those mobilised and circulating in AMI did not.
循环CD34(+)CD133(+)细胞是循环内皮祖细胞（EPC）的主要来源之一。在稳定型冠状动脉疾病（CAD）中，年龄与CD34(+)CD133(+)祖细胞的数量和功能呈负相关，但在急性心肌梗死（AMI）中这种关系仍不明确。作者旨在阐明衰老如何影响AMI中动员的CD34(+)CD133(+)祖细胞的数量和功能。
通过流式细胞术检测循环CD34(+)CD133(+)祖细胞。在CAD患者入院时或AMI发病后第7天进行检测。在稳定型CAD患者（n = 131）中，循环CD34(+)CD133(+)细胞数量随年龄增长而减少（r = -0.344，p < 0.0001）。在AMI患者中（n = 50），循环CD34(+)CD133(+)细胞数量与年龄无相关性，多因素分析显示循环CD34(+)CD133(+)细胞数量减少与男性性别及较高的肌酸激酶峰值相关。与AMI患者（N = 6）相比，稳定型CAD患者（n = 10）中具有良好迁移和管形成能力的功能性EPC的产生能力下降。
在稳定型CAD中，循环CD34(+)CD133(+)祖细胞的数量和功能随年龄增长而下降，而在AMI中动员并循环的这些细胞数量和功能则不然。
