Xiao Cheng, Liu Lixiang, Li Xi, Yang Xiaoan, Liu Hanxiong
Medical College, Hunan Polytechnic of Environment and Biology, Hengyang 421005, China.
Department of Gynecology, The Seventh Affiliated Hospital, Sun Yat-Sen University, Shenzhen 518107, China.
Cardiol Res Pract. 2022 Jul 5;2022:9619626. doi: 10.1155/2022/9619626. eCollection 2022.
The number and function of circulating endothelial progenitor cells (EPCs) decreased in stable coronary artery disease. Nevertheless, there were few studies that explored the variation of EPC and the relationship with the severity of coronary artery lesions in unstable angina (UA). Therefore, this leaves an area for the investigation of the difference in the number and activity of circulating EPCs and the relationship with the Gensini score in unstable angina. Fluorescence-activated cell sorter analysis, as well as DiI-acLDL and lectin fluorescent staining measure the number of circulating EPCs. The transwell chamber assay and MTT were evaluated by the migration and proliferation of circulating EPCs. In addition, the flow-mediated dilation (FMD), Gensini score, and IL-6 levels in plasma were determined. We found that UA patients had the higher number and lower function of circulating EPCs. With the increase in severity of coronary artery lesions, the migration and proliferation of EPCs were decreased. Moreover, the function of the circulating EPCs was negatively associated with severity of coronary artery lesions in unstable angina. In addition, UA patients presented elevated IL-6, which was negatively correlated with the function of circulating EPCs and FMD and positively correlated with the severity of coronary artery lesions evaluated by the Gensini score. These findings revealed the decline in the function of circulating EPCs was associated with the severity of coronary artery disease, which may be related to systemic inflammation.
在稳定型冠状动脉疾病中，循环内皮祖细胞（EPCs）的数量和功能会降低。然而，很少有研究探讨不稳定型心绞痛（UA）中EPCs的变化及其与冠状动脉病变严重程度的关系。因此，这就留下了一个研究不稳定型心绞痛中循环EPCs数量和活性差异及其与Gensini评分关系的领域。荧光激活细胞分选分析以及DiI - acLDL和凝集素荧光染色用于测量循环EPCs的数量。通过循环EPCs的迁移和增殖来评估Transwell小室测定法和MTT。此外，还测定了血流介导的血管舒张（FMD）、Gensini评分和血浆中的IL - 6水平。我们发现UA患者循环EPCs的数量较多但功能较低。随着冠状动脉病变严重程度的增加，EPCs的迁移和增殖减少。此外，不稳定型心绞痛中循环EPCs的功能与冠状动脉病变严重程度呈负相关。另外，UA患者的IL - 6升高，其与循环EPCs的功能和FMD呈负相关，与通过Gensini评分评估的冠状动脉病变严重程度呈正相关。这些发现揭示了循环EPCs功能的下降与冠状动脉疾病的严重程度相关，这可能与全身炎症有关。
