Kawchuk L M, Martin R R, Rochon D M, McPherson J
Research Station, Agriculture Canada, Vancouver, British Columbia.
J Gen Virol. 1989 Mar;70 ( Pt 3):783-8. doi: 10.1099/0022-1317-70-3-783.
Complementary DNA clones representing approximately 6100 nucleotides of potato leafroll virus (PLRV) were generated, restriction-mapped, and partially sequenced. Within one of the cDNA clones an open reading frame (ORF) encoding a 23K protein was identified and further characterized. Amino acid sequence comparison of this protein showed significant homology (47.1%) with the barley yellow dwarf virus (BYDV-PAV) coat protein. This and other observations suggested that this gene encodes the PLRV coat protein. Other similarities were observed between PLRV and BYDV sequences in this region of their genomes, including an ORF of 17K within the ORF encoding the 23K putative coat protein.
生成了代表马铃薯卷叶病毒（PLRV）约6100个核苷酸的互补DNA克隆，进行了限制性图谱分析并部分测序。在其中一个cDNA克隆中鉴定出一个编码23K蛋白的开放阅读框（ORF）并进一步进行了表征。该蛋白的氨基酸序列比较显示与大麦黄矮病毒（BYDV-PAV）外壳蛋白具有显著同源性（47.1%）。这一发现及其他观察结果表明该基因编码PLRV外壳蛋白。在PLRV和BYDV基因组的这一区域序列中还观察到其他相似之处，包括在编码23K假定外壳蛋白的ORF内有一个17K的ORF。
