Vincent J R, Ueng P P, Lister R M, Larkins B A
Department of Botany and Plant Pathology, Purdue University, West Lafayette, Indiana 47907.
J Gen Virol. 1990 Dec;71 ( Pt 12):2791-9. doi: 10.1099/0022-1317-71-12-2791.
Barley yellow dwarf virus (BYDV) can be separated into two groups based on, among other criteria, serological relationships that are presumably governed by the viral capsid structure. Nucleotide sequences for the coding regions of coat proteins of approximately 22 K were identified for the MAV-PS1, P-PAV (group 1) and NY-RPV (group 2) isolates of BYDV. The MAV-PS1 and P-PAV coat protein sequences shared 71% deduced amino acid similarity whereas that of the NY-RPV isolate shared no more than 51% similarity with either the MAV-PS1 or the P-PAV sequence. Other comparisons showed that these and other BYDV coat protein sequences examined to date share a high degree of identity with those identified from other luteoviruses. Among luteovirus coat protein sequences in general, several highly conserved domains were identified whereas other domains differentiate MAV-PS1 and PAV isolates from NY-RPV and other luteoviruses. Sequence similarities and differences among BYDV coat proteins (approx. 22K) are consistent with the serological relationships exhibited by these viruses. Amino acid sequence comparisons between BYDV isolates that share common aphid vectors indicate that it is unlikely that these coat proteins are involved in aphid specificity.
大麦黄矮病毒（BYDV）可根据多种标准进行分类，其中包括推测由病毒衣壳结构决定的血清学关系，据此可分为两组。已确定了BYDV的MAV-PS1、P-PAV（第1组）和NY-RPV（第2组）分离株约22K的外壳蛋白编码区的核苷酸序列。MAV-PS1和P-PAV外壳蛋白序列推导的氨基酸相似性为71%，而NY-RPV分离株的序列与MAV-PS1或P-PAV序列的相似性不超过五1%。其他比较表明，迄今检测的这些BYDV外壳蛋白序列以及其他序列与从其他黄症病毒鉴定的序列具有高度同一性。一般而言，在黄症病毒外壳蛋白序列中，鉴定出了几个高度保守的结构域，而其他结构域则将MAV-PS1和PAV分离株与NY-RPV及其他黄症病毒区分开来。BYDV外壳蛋白（约22K）之间的序列异同与这些病毒呈现的血清学关系一致。对共有常见蚜虫传播介体的BYDV分离株进行的氨基酸序列比较表明，这些外壳蛋白不太可能与蚜虫特异性有关。
