Liñeiro Eva, Chiva Cristina, Cantoral Jesús M, Sabidó Eduard, Fernández-Acero Francisco Javier
Andalusian Center for Grape and Grapevine Research (IVAGRO), CeiA3, Marine and Environmental Sciences Faculty, University of Cadiz, Puerto Real, Spain.
Proteomics Unit, Centre for Genomic Regulation (CRG), Barcelona, Spain.
Proteomics. 2016 Sep;16(17):2363-76. doi: 10.1002/pmic.201500496. Epub 2016 Aug 5.
Botrytis cinerea is a model fungus for the study of phytopathogenicity that exhibits a wide arsenal of tools to infect plant tissues. Most of these factors are related to signal transduction cascades, in which membrane proteins play a key role as a bridge between environment and intracellular molecular processes. This work describes the first description of the membranome of Botrytis under different pathogenicity conditions induced by different plant-based elicitors: glucose and tomato cell wall (TCW). A discovery proteomics analysis of membrane proteins was carried out by mass spectrometry. A total of 2794 proteins were successfully identified, 46% of them were classified as membrane proteins based on the presence of transmembrane regions and lipidation. Further analyses showed significant differences in the membranome composition depending on the available carbon source: 804 proteins were exclusively identified when the fungus was cultured with glucose as a sole carbon source, and 251 proteins were exclusively identified with TCW. Besides, among the 1737 common proteins, a subset of 898 proteins presented clear differences in their abundance. GO enrichment and clustering interaction analysis revealed changes in the composition of membranome with increase of signalling function in glucose conditions and carbohydrate degradation process in TCW conditions. All MS data have been deposited in the ProteomeXchange with identifier PXD003099 (http://proteomecentral.proteomexchange.org/dataset/PXD003099).
灰葡萄孢是用于研究植物致病性的模式真菌，它具有多种感染植物组织的工具。这些因素大多与信号转导级联反应有关，其中膜蛋白作为环境与细胞内分子过程之间的桥梁发挥着关键作用。这项工作首次描述了在不同植物来源的激发子（葡萄糖和番茄细胞壁（TCW））诱导的不同致病条件下灰葡萄孢的膜蛋白质组。通过质谱对膜蛋白进行了发现蛋白质组学分析。共成功鉴定出2794种蛋白质，其中46%基于跨膜区域和脂化的存在被归类为膜蛋白。进一步分析表明，根据可用碳源的不同，膜蛋白质组的组成存在显著差异：当真菌以葡萄糖作为唯一碳源培养时，仅鉴定出804种蛋白质，而以TCW培养时仅鉴定出251种蛋白质。此外，在1737种常见蛋白质中，有898种蛋白质的丰度存在明显差异。基因本体论（GO）富集和聚类相互作用分析表明，在葡萄糖条件下，膜蛋白质组的组成随着信号功能的增加而发生变化，在TCW条件下则随着碳水化合物降解过程而变化。所有质谱数据已存入蛋白质组交换库，标识符为PXD003099（http://proteomecentral.proteomexchange.org/dataset/PXD003099）。
