Division of Neurosurgical Research, Medical Faculty Mannheim, Heidelberg University, Mannheim, D-68167, Germany.
Department of Clinical Pharmacy &Pharmacology, University Medical Center Groningen, University of Groningen, 9700 RB, The Netherlands.
Sci Rep. 2016 Jun 22;6:28401. doi: 10.1038/srep28401.
Occlusion of the middle cerebral artery (MCA) by an intraluminal filament is widely used to study focal brain ischemia in male Sprague-Dawley rats. However, permanent occlusion goes along with a high fatality. To overcome this drawback we designed a new filament carrying a bowling pin-shaped tip (BP-tip) and compared this with three conventionally tipped filaments. Follow-up periods were 24 h (all groups) and 72 and 120 h in BP-tip group. Ischemic damage and swelling were quantified using silver nitrate staining. Collateral flow via the posterior cerebral artery (PCA) was assessed using selective dye perfusion of the internal carotid artery. Despite a comparable decrease of brain perfusion in all groups, ischemic damage was significantly smaller in BP-tips (p < 0.05). Moreover, BP-tip significantly reduced mortality from 60% to 12.5% and widely spared the occipital region and hypothalamus from ischemic damage. Conventional but not BP-tip filaments induced vascular distortion, measured as gross displacement of the MCA origin, which correlated with occipital infarction size. Accordingly, BP-tip occluded rats showed a significantly better collateral filling of the PCA territory. Ischemic volume significantly increased in BP-tip occlusion at 72 h follow-up. BP-tip filaments offer superior survival in permanent MCA occlusion, while mimicking the course of a malignant stroke in patients.
大脑中动脉（MCA）腔内栓子阻塞广泛用于雄性 Sprague-Dawley 大鼠局灶性脑缺血的研究。然而，永久性闭塞伴随着高死亡率。为了克服这一缺点，我们设计了一种带有保龄球钉状尖端（BP 尖端）的新型纤维，并将其与三种常规尖端纤维进行了比较。随访时间为 24 h（所有组）和 BP 尖端组的 72 和 120 h。使用硝酸银染色定量缺血性损伤和肿胀。通过颈内动脉选择性染料灌注评估大脑后动脉（PCA）的侧支血流。尽管所有组的脑灌注均有可比的降低，但 BP 尖端组的缺血性损伤明显较小（p < 0.05）。此外，BP 尖端将死亡率从 60%显著降低至 12.5%，并广泛避免了枕叶和下丘脑免受缺血性损伤。常规但不是 BP 尖端的纤维诱导血管扭曲，表现为 MCA 起源的明显移位，这与枕叶梗死的大小相关。因此，BP 尖端闭塞的大鼠 PCA 区域的侧支充盈明显更好。BP 尖端闭塞在 72 h 随访时，缺血性体积显著增加。BP 尖端纤维在永久性 MCA 闭塞中提供了更好的生存机会，同时模拟了患者恶性中风的过程。
