Liu Shaoying, Fan Jicai, Huang Xihui, Jin Quan, Zhu Guonian
Hangzhou Center for Disease Control and Prevention, Laboratory of Chemistry and Physics, Mingshi Rd No. 568, Hangzhou 310021, People's Republic of China.
Zhejiang University, Institute of Pesticide and Environmental Toxicology, Yu Hang Tang Rd No. 866, Hangzhou, 310029, People's Republic of China.
J AOAC Int. 2016 Sep;99(5):1273-8. doi: 10.5740/jaoacint.16-0098. Epub 2016 Jun 21.
A simple method was developed for the determination of sterigmatocystin in infant cereals. The method consists of a quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction approach and cleanup with an SPE HLB cartridge. After purification for separation and quantification of sterigmatocystin, HPLC with UV detection and diode-array detection (DAD) was used at sterigmatocystin levels >10 μg/kg, whereas ultra-performance LC with electrospray ionization (ESI) and tandem MS (MS/MS) was used at levels <10 μg/kg. The compound was determined by UV-DAD at 325 nm and by ESI-MS/MS in the positive ionization mode. Good r(2) values (≥0.99) were found with both UV-DAD and ESI-MS/MS, and satisfactory recoveries (84.8-96.2%) of sterigmatocystin in infant cereals were demonstrated, with RSD values ≤11.43%. The developed analytical method was used for the determination of sterigmatocystin in infant cereals from Hangzhou, China.
开发了一种用于测定婴幼儿谷物中杂色曲霉素的简单方法。该方法包括快速、简便、廉价、高效、耐用且安全的（QuEChERS）萃取方法，并用固相萃取HLB柱进行净化。在对杂色曲霉素进行分离和定量的净化之后，当杂色曲霉素水平>10μg/kg时，使用配有紫外检测和二极管阵列检测（DAD）的高效液相色谱法，而当水平<10μg/kg时，则使用配有电喷雾电离（ESI）和串联质谱（MS/MS）的超高效液相色谱法。该化合物通过在325nm处的紫外-DAD以及在正离子模式下的ESI-MS/MS进行测定。紫外-DAD和ESI-MS/MS均具有良好的r(2)值（≥0.99），并且证明了婴幼儿谷物中杂色曲霉素的回收率令人满意（84.8-96.2%），相对标准偏差值≤11.43%。所开发的分析方法用于测定中国杭州的婴幼儿谷物中的杂色曲霉素。
