Basu Aakash, Lagier Samuel, Vologodskaia Maria, Fabella Brian A, Hudspeth A J
Laboratory of Sensory Neuroscience, Howard Hughes Medical Institute, The Rockefeller University, New York, United States.
Elife. 2016 Jun 22;5:e16041. doi: 10.7554/eLife.16041.
Mechanoelectrical transduction by hair cells commences with hair-bundle deflection, which is postulated to tense filamentous tip links connected to transduction channels. Because direct mechanical stimulation of tip links has not been experimentally possible, this hypothesis has not been tested. We have engineered DNA tethers that link superparamagnetic beads to tip links and exert mechanical forces on the links when exposed to a magnetic-field gradient. By pulling directly on tip links of the bullfrog's sacculus we have evoked transduction currents from hair cells, confirming the hypothesis that tension in the tip links opens transduction channels. This demonstration of direct mechanical access to tip links additionally lays a foundation for experiments probing the mechanics of individual channels.
毛细胞的机械电转导始于毛束偏转，据推测这会使连接到转导通道的丝状纤毛顶端连接紧张。由于尚未通过实验直接对顶端连接进行机械刺激，该假设尚未得到验证。我们设计了DNA系链，将超顺磁珠连接到顶端连接，并在暴露于磁场梯度时对连接施加机械力。通过直接拉动牛蛙球囊的顶端连接，我们诱发了毛细胞的转导电流，证实了顶端连接中的张力会打开转导通道这一假设。这种对顶端连接进行直接机械操作的演示，还为探究单个通道力学的实验奠定了基础。
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