Effect of amogastrin on the release of various prostaglandins into gastric lumen in man.

The effect of amogastrin on the release of 5 types of prostaglandins (PGs) [PGE2, 6-keto-PGF1 alpha, PGF2 alpha, PGD2, and thromboxan B2 (TXB2)] into the gastric lumen was evaluated in seven healthy volunteers. Gastric juice was obtained under basal and amogastrin-stimulated conditions. The 5 types of PGs in the gastric juice were separated by high-performance liquid chromatography and then measured by radioimmunoassay. PGE2 was the major component in gastric juices among the PGs measured in this study. The release of 5 types of PGs increased after amogastrin administration. The rate of increase was high for PGE2 and 6-keto-PGF1 alpha (3.48 and 3.98 respectively), whereas it was low for PGD2 (1.54). It is suggested that endogenously synthesized PGs, especially PGE2 and PGI2 (assayed as 6-keto-PGF1 alpha), play important roles in the gastric mucosal protection and the regulation of acid secretion.