Key Laboratory of Advanced Technologies of Materials, Ministry of Education of China, School of Materials Science and Engineering, Southwest Jiaotong University , Chengdu, 610031, China.
Beijing Engineering Research Center for BioNanotechnology and CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for NanoScience and Technology , 11 Beiyitiao, ZhongGuanCun, Beijing, 100190, China.
Biomacromolecules. 2016 Jul 11;17(7):2472-8. doi: 10.1021/acs.biomac.6b00642. Epub 2016 Jun 28.
Nanocrystalline cellulose (NCC) is a kind of natural biopolymers with merits of large surface area, high specific strength and unique optical properties. This report shows that NCC can serve as the substrate, allowing glucose to reduce Tollen's reagent to produce silver nanoparticles (AgNPs) at room temperature. The generation of AgNPs is affected by the factors such as the concentrations of silver ions, NCC and glucose, as well as the different reaction temperatures. The AgNPs with NCC are applied for the development of a visual, quantitative, nonenzymatic and high-sensitive assay for glucose detection in serum. This assay is also used for monitoring the concentration change of glucose in medium during cell culture. For the antibacterial activity, the minimal inhibitory concentration (MIC) of the generated AgNPs with NCC is much lower than that of commercial AgNPs, attributed to the good dispersion of AgNPs with the presence of NCC. As NCC exhibits unique advantages including green, stable, inexpensive, and abundant, the NCC-based generation of AgNPs may find promising applications in clinical diagnosis, environmental monitoring, and the control of bacteria.
纳米纤维素（NCC）是一种天然生物聚合物，具有比表面积大、比强度高和独特光学性能等优点。本报告表明，NCC 可以作为基质，使葡萄糖在室温下将托伦试剂还原为银纳米粒子（AgNPs）。AgNPs 的生成受银离子、NCC 和葡萄糖浓度以及不同反应温度等因素的影响。利用负载 NCC 的 AgNPs 开发了一种用于检测血清中葡萄糖的可视化、定量、非酶和高灵敏度的方法。该方法还用于监测细胞培养过程中培养基中葡萄糖浓度的变化。在抗菌活性方面，负载 NCC 的 AgNPs 的最小抑菌浓度（MIC）明显低于商用 AgNPs，这归因于 NCC 的存在使 AgNPs 具有良好的分散性。由于 NCC 具有绿色、稳定、廉价和丰富等独特优势，基于 NCC 的 AgNPs 的生成可能在临床诊断、环境监测和细菌控制方面有很好的应用前景。
